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Team:DTU-Denmark/Notebook/1 July 2013
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*10 mL Casamino Acid ( ready solution from Andreas) | *10 mL Casamino Acid ( ready solution from Andreas) | ||
| - | *2 mM | + | *2 mM MgSO<sub>4</sub> (from 2M liquid stock) |
| - | *0.1 mM | + | *0.1 mM CaCl<sub>2</sub> (liquid solution with 15% gly) |
*200 mL M9 salts | *200 mL M9 salts | ||
* Fill to 1 L with sterilized water | * Fill to 1 L with sterilized water | ||
1 L stock of M9 salts: | 1 L stock of M9 salts: | ||
| - | * 64 g | + | * 64 g Na<sub>2</sub>HPO<sub>4</sub>*7H<sub>2</sub>O = 31.2 g Na2HPO4 |
| - | * 15 g | + | * 15 g KH<sub>2</sub>PO<sub>4</sub> |
* 25 g NaCl | * 25 g NaCl | ||
| - | *5 g | + | *5 g NH<sub>4</sub>Cl |
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{{:Team:DTU-Denmark/Templates/EndPage}} | {{:Team:DTU-Denmark/Templates/EndPage}} | ||
Latest revision as of 11:13, 4 October 2013
1 July 2013
Contents |
208 lab
Main purpose today
Make M9 Medium for the biolector toxicity experiment
Who was in the lab
Helen, Gosia, Ariadni
Procedure
According to open wetware protocol for Biolector Experiment
1L of Media:
- 10 mL Casamino Acid ( ready solution from Andreas)
- 2 mM MgSO4 (from 2M liquid stock)
- 0.1 mM CaCl2 (liquid solution with 15% gly)
- 200 mL M9 salts
- Fill to 1 L with sterilized water
1 L stock of M9 salts:
- 64 g Na2HPO4*7H2O = 31.2 g Na2HPO4
- 15 g KH2PO4
- 25 g NaCl
- 5 g NH4Cl
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