Team:DTU-Denmark/Notebook/1 July 2013

From 2013.igem.org

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(Procedure)
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{{:Team:DTU-Denmark/Templates/StartPage|1 July 2013}}
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=208 lab=
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<hr/>
== Main purpose today ==
== Main purpose today ==
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<hr/>
Make M9 Medium for the biolector toxicity experiment
Make M9 Medium for the biolector toxicity experiment
==Who was in the lab==
==Who was in the lab==
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<hr/>
Helen, Gosia, Ariadni
Helen, Gosia, Ariadni
==Procedure==
==Procedure==
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<hr/>
According to open wetware protocol for Biolector Experiment
According to open wetware protocol for Biolector Experiment
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*10 mL Casamino Acid ( ready solution from Andreas)
*10 mL Casamino Acid ( ready solution from Andreas)
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*2 mM MgSO4 (from 2M liquid stock)
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*2 mM MgSO<sub>4</sub> (from 2M liquid stock)
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*0.1 mM CaCl2 (liquid solution with 15% gly)
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*0.1 mM CaCl<sub>2</sub> (liquid solution with 15% gly)
*200 mL M9 salts
*200 mL M9 salts
* Fill to 1 L with sterilized water
* Fill to 1 L with sterilized water
1 L stock of M9 salts:
1 L stock of M9 salts:
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* 64 g Na2HPO4*7H2O = 31.2 g Na2HPO4
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* 64 g Na<sub>2</sub>HPO<sub>4</sub>*7H<sub>2</sub>O = 31.2 g Na2HPO4
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* 15 g KH2PO4
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* 15 g KH<sub>2</sub>PO<sub>4</sub>
* 25 g NaCl
* 25 g NaCl
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*5 g NH4Cl
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*5 g NH<sub>4</sub>Cl
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{{:Team:DTU-Denmark/Templates/EndPage}}

Latest revision as of 11:13, 4 October 2013

1 July 2013

Contents

208 lab


Main purpose today


Make M9 Medium for the biolector toxicity experiment

Who was in the lab


Helen, Gosia, Ariadni

Procedure


According to open wetware protocol for Biolector Experiment

1L of Media:

  • 10 mL Casamino Acid ( ready solution from Andreas)
  • 2 mM MgSO4 (from 2M liquid stock)
  • 0.1 mM CaCl2 (liquid solution with 15% gly)
  • 200 mL M9 salts
  • Fill to 1 L with sterilized water

1 L stock of M9 salts:

  • 64 g Na2HPO4*7H2O = 31.2 g Na2HPO4
  • 15 g KH2PO4
  • 25 g NaCl
  • 5 g NH4Cl

Navigate to the Previous or the Next Entry