Team:DTU-Denmark/Notebook/28 June 2013
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Make PCR with x7-polymerase. | Make PCR with x7-polymerase. | ||
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Kristian | Kristian |
Latest revision as of 18:08, 30 September 2013
28 June 2013
Contents |
208 lab
Main purposes today
Make PCR with x7-polymerase.
Who was in the lab
Kristian
Procedure
Made PCRs on pZA21, GFP SF TAT, GFP SF Sec, RFP all samples where made in duplicates.
In tube 1+2, 9+10 where pZA21. In 3+4, 11+12 GFP SF TAT In 5+6, 13+14, 17+18 GFP SF Sec In 7+8, 15+16 RFP
First program was 59°C annealing and 2:00 extension time with tube: 1+2, 3+4, 5+6, 7+8. Second program was 68°C annealing and 2:00 extension time with tube: 9+10, 11+12, 13+14, 15+16. Last program was 70°C annealing and 1:00 extension time with tube: 17+18.
Results
Conclusion from today
We might have used the wrong polymerase and the products we have gotten from previous PCRs with PHUSION-polymerase are without any uracil insertion.
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