Latest revision as of 12:12, 4 October 2013

8 September

lab 208

Who was in the lab

Henrike, Kristian, Julia

Procedure

Colony PCR to verify Nir in pZA21::ara-tight

Colony PCR on the Nir transformants, testing for NirH (primers 45a, 45b, annealing 59C), NirM+S (primers 44, 11a annealing 64C) and NirN (primers 43, 11b annealing 68C).

program:

temperature	time	cycles
98C	10:00	-
98C	0:10	36
59C/64C/68C	0:30	36
72C	2:00	36
72C	5:00	-
10C	hold	-

Colony PCR to verify HAO insert.

Primers: 28a, 28b

temperature: 59C

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@@ Line 2: / Line 2: @@
 =lab 208=
-<hr/>
-==Main purpose==
 <hr/>
 ==Who was in the lab==
 <hr/>
+Henrike, Kristian, Julia
 ==Procedure==
@@ Line 14: / Line 13: @@
 ===Colony PCR to verify Nir in pZA21::ara-tight===
-==Results==
+Colony PCR on the Nir transformants, testing for NirH (primers 45a, 45b, annealing 59C), NirM+S (primers 44, 11a annealing 64C) and NirN (primers 43, 11b annealing 68C).
-<hr/>
+program:
+{| class="wikitable" style="text-align: right"
+! temperature !! time !! cycles
+|-
+|  98C || 10:00 || -
+|-
+|  98C || 0:10 || 36
+|-
+|  59C/64C/68C || 0:30 || 36
+|-
+|  72C || 2:00 || 36
+|-
+|  72C || 5:00 || -
+|-
+|  10C || hold || -
+|-
+|}
+Colony PCR to verify HAO insert.
+Primers: 28a, 28b
+temperature: 59C
-Navigate to the [[Team:DTU-Denmark/Notebook/7_September_2013|Previous]] or the [[Team:DTU-Denmark/Notebook/9_September_20133|Next]] Entry
+Navigate to the [[Team:DTU-Denmark/Notebook/7_September_2013|Previous]] or the [[Team:DTU-Denmark/Notebook/9_September_2013|Next]] Entry
 {{:Team:DTU-Denmark/Templates/EndPage}}

Team:DTU-Denmark/Notebook/8 September 2013

From 2013.igem.org