Team:DTU-Denmark/Notebook/9 September 2013

From 2013.igem.org

(Difference between revisions)
(→Transfomation)
Line 31: Line 31:
*negative Nir in pZA21::ara tight - no colonies
*negative Nir in pZA21::ara tight - no colonies
 +
 +
===Gel===
 +
 +
Gel on the results of the first colony PCR on the Nir transformants, testing for NirH, NirM+S and NirN
 +
 +
* 1 kb ladder
 +
* colony 1, NirH
 +
* colony 1, Nir M+S
 +
* colony 1, NirN
 +
* colony 2, NirH
 +
* colony 2, Nir M+S
 +
* colony 2, NirN
 +
* colony 3, NirH
 +
* colony 3, Nir M+S
 +
* colony 3, NirN
 +
* colony 4, NirH
 +
* colony 4, Nir M+S
 +
* colony 4, NirN
 +
* 1 kb ladder
 +
 +
The gel picture is inconclusive. We will do another colony PCR using the primers designed for sequencing.
Navigate to the [[Team:DTU-Denmark/Notebook/8_September_2013|Previous]] or the [[Team:DTU-Denmark/Notebook/10_September_20133|Next]] Entry
Navigate to the [[Team:DTU-Denmark/Notebook/8_September_2013|Previous]] or the [[Team:DTU-Denmark/Notebook/10_September_20133|Next]] Entry
{{:Team:DTU-Denmark/Templates/EndPage}}
{{:Team:DTU-Denmark/Templates/EndPage}}

Revision as of 10:11, 11 September 2013

9 September

Contents

lab 208

Main purpose

Who was in the lab

Julia, Kristian, Henrike

Procedure

Colony PCR to verify inserts HAO and Nir in pZA21::ara and pZA21::ara tight

Results

Transfomation

Transformation on last friday gave the following results:

  • HAO in pZA21::ara (from Biobrick K808000) - 6 colonies (3 orange and small, 3 normal color and very big)
  • negative HAO in pZA21::ara (from Biobrick K808000) - no colonies
  • HAO in pZA21::ara tight - a lot of colonies, some very big
  • negative HAO in pZA21::ara tight - a lot of small colonies
  • Nir in pZA21::ara tight - 8 colonies
  • negative Nir in pZA21::ara tight - no colonies


Gel

Gel on the results of the first colony PCR on the Nir transformants, testing for NirH, NirM+S and NirN

  • 1 kb ladder
  • colony 1, NirH
  • colony 1, Nir M+S
  • colony 1, NirN
  • colony 2, NirH
  • colony 2, Nir M+S
  • colony 2, NirN
  • colony 3, NirH
  • colony 3, Nir M+S
  • colony 3, NirN
  • colony 4, NirH
  • colony 4, Nir M+S
  • colony 4, NirN
  • 1 kb ladder

The gel picture is inconclusive. We will do another colony PCR using the primers designed for sequencing.

Navigate to the Previous or the Next Entry

Retrieved from "http://2013.igem.org/Team:DTU-Denmark/Notebook/9_September_2013"