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Team:DTU-Denmark/Notebook/9 September 2013
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The gel picture is inconclusive. We will do another colony PCR using the primers designed for sequencing. | The gel picture is inconclusive. We will do another colony PCR using the primers designed for sequencing. | ||
Revision as of 10:12, 11 September 2013
9 September
Contents |
lab 208
Main purpose
Who was in the lab
Julia, Kristian, Henrike
Procedure
Colony PCR to verify inserts HAO and Nir in pZA21::ara and pZA21::ara tight
Results
Transfomation
Transformation on last friday gave the following results:
- HAO in pZA21::ara (from Biobrick K808000) - 6 colonies (3 orange and small, 3 normal color and very big)
- negative HAO in pZA21::ara (from Biobrick K808000) - no colonies
- HAO in pZA21::ara tight - a lot of colonies, some very big
- negative HAO in pZA21::ara tight - a lot of small colonies
- Nir in pZA21::ara tight - 8 colonies
- negative Nir in pZA21::ara tight - no colonies
Gel
Gel on the results of the first colony PCR on the Nir transformants, testing for NirH, NirM+S and NirN
- 1 kb ladder
- colony 1, NirH
- colony 1, Nir M+S
- colony 1, NirN
- colony 2, NirH
- colony 2, Nir M+S
- colony 2, NirN
- colony 3, NirH
- colony 3, Nir M+S
- colony 3, NirN
- colony 4, NirH
- colony 4, Nir M+S
- colony 4, NirN
- 1 kb ladder
The gel picture is inconclusive. We will do another colony PCR using the primers designed for sequencing.
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