Team:DTU-Denmark/Notebook/9 September 2013

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The gel picture is inconclusive. We will do another colony PCR using the primers designed for sequencing.
The gel picture is inconclusive. We will do another colony PCR using the primers designed for sequencing.
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Revision as of 11:58, 11 September 2013

9 September

Contents

lab 208


Main purpose


Who was in the lab


Julia, Kristian, Henrike

Procedure


Colony PCR to verify inserts HAO and Nir in pZA21::ara and pZA21::ara tight

Results


Transfomation

Transformation on last friday gave the following results:

  • HAO in pZA21::ara (from Biobrick K808000) - 6 colonies (3 orange and small, 3 normal color and very big)
  • negative HAO in pZA21::ara (from Biobrick K808000) - no colonies
  • HAO in pZA21::ara tight - a lot of colonies, some very big
  • negative HAO in pZA21::ara tight - a lot of small colonies
  • Nir in pZA21::ara tight - 8 colonies
  • negative Nir in pZA21::ara tight - no colonies


Gel

Gel on the results of the first colony PCR on the Nir transformants, testing for NirH, NirM+S and NirN

  • 1 kb ladder
  • colony 1, NirH
  • colony 1, Nir M+S
  • colony 1, NirN
  • colony 2, NirH
  • colony 2, Nir M+S
  • colony 2, NirN
  • colony 3, NirH
  • colony 3, Nir M+S
  • colony 3, NirN
  • colony 4, NirH
  • colony 4, Nir M+S
  • colony 4, NirN
  • 1 kb ladder

2013-09-09 nir col.jpg

The gel picture is inconclusive. We will do another colony PCR using the primers designed for sequencing.

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