It turns out that the structures of some of the proteins we want our mutants to express have been determined.
Mutant 1
Hao
Hydroxylamine oxidoreductase
source 1FGJ
cc554
Cytochrome c554
source 1FT5
Mutant 2
NirS
Nitrite reductase
NirS dimer with N2 - source 1NNO