"
Team:DTU-Denmark/Protein Models
From 2013.igem.org
Protein Models
It turns out that the structures of some of the proteins we want our mutants to express have been determined.
Contents |
Mutant 1
AMO
Ammonia monooxygenase
homology models of all three subunits of AMO
At the time of writing neither of the the three subunits from Nitrosomonas Europae is available as 3D structures, so each of the subunits was identified at UNIPROT, and their sequence was homologymodelled using CPHmodels-3.2
| Resource | AmoA | AmoB | AmoC |
|---|---|---|---|
| Uniprot | Q04507 | Q04508 | Q82T63 |
| Template pdb code and chain | 1YEW.A | 1YEW.B | 3RGB.C |
| Coverage | 90.5 | 86.2 | 66.4 |
| E-value | 1e-87 | 3e-67 | 8e-43 |
The three subunits were combined and aligned to the subunits of the Crystal structure of particulate methane monooxygenase, hence an overlap of two alpha helices.
Hao
Hydroxylamine oxidoreductase
source 1FGJ
cc554
Cytochrome c554
source 1FT5
| Resource | Ccm552 |
|---|---|
| Uniprot | Q50926 |
| Template pdb code | 2J7A |
| Coverage | 68.3 |
| E-value | 6e-11 |
Mutant 2
NirS
Nitrite reductase
As a dimer with N2 - source 1NNO
All models were made and visualized in PyMOL v1.6
