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Sensing

Making a glycerol stock
From each culture that was inoculated we made a 50% glycerol stock of 500ul Bacterial culture and 500ul 50% glycerol.

PCR
We Started the Gibson assembly of four of our constructs by making a PCR of each, the Insert as well as the Backbone, making eight different PCR tubes in total (see protocol PCR). About twenty minutes before the PCR reaction was done, the machine malfunctionned. We did run a 0.8% gel, but The results cannot be well interpreted, as the reaction could not go to completion. We re-ran another PCR reaction with the same constructs, as well as a reactant control. After the PCR the amplified DNA was stored over night at 4°C.

Tubes: 1.) MMP2 insert with linker(5.4-I)
2.) MMP2 insert without linker (4.4-I)
3.) Backbone with GFP (5.4-B)
4.) Backbone without GFP (4.4-B)
1.) MMP9 insert with linker(7.4-I)
2.) MMP9 insert without linker (6.4-I)
3.) Backbone with GFP (7.4-B)
4.) Backbone without GFP (6.4-B)