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Sensing-Effector

Sequencing of the hya promoter plasmid
-I send the newly isolated and purified hya-containing plasmid for sequencing.

Transformation
-Because the functional assays of the sensing module were inconclusive, I decided to do another transformation with the Plasmids that I had sent for sequencing and whose results showed that the promoters were really present, namely the cad-promoter and the constitutive promoter from iGEM.