Team:EPF Lausanne/Calendar/24 July 2013


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Taxi.Coli: Smart Drug Delivery iGEM EPFL


Cell Surface Display
Our Primers arrived today.

We did a PCR (see protocols) to amplify pINP_construct to first remove the EYFP sequence and by the same way add overhangs for the Gibson reaction. We thus did a 0.8% Gel electrophoresis to verify the PCR product. The PCR needed to be optimised.


Competent cells try 2.