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''PCR optimisation:'' to improve the ''pINP_construct'' amplification we did a gradient PCR at 72, 73 and 74°C with 3% and 5% DMSO added to the mix. | ''PCR optimisation:'' to improve the ''pINP_construct'' amplification we did a gradient PCR at 72, 73 and 74°C with 3% and 5% DMSO added to the mix. | ||
we verified our results with a 0.8% gel electrophoresis. | we verified our results with a 0.8% gel electrophoresis. | ||
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| + | '''General''' | ||
| + | |||
| + | Competent cells try 3: it worked! | ||
| + | |||
{{Template:EPFL2013Footer}} | {{Template:EPFL2013Footer}} | ||
PCR optimisation: to improve the pINP_construct amplification we did a gradient PCR at 72, 73 and 74°C with 3% and 5% DMSO added to the mix. we verified our results with a 0.8% gel electrophoresis.
General
Competent cells try 3: it worked!
"
