Team:EPF Lausanne/Calendar/25 July 2013

From 2013.igem.org

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'''Cell Surface Display'''
'''Cell Surface Display'''
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''PCR optimisation:'' to improve the ''pINP_construct'' amplification we did a gradient PCR at 72, 73 and 74°C with 3% and 5% DMSO added to the mix.  
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''PCR optimisation'' <BR>
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To improve the ''pINP_construct'' amplification we did a gradient PCR at 72°C, 73°C and 74°C with 3% and 5% DMSO added to the mix.  
we verified our results with a 0.8% gel electrophoresis.
we verified our results with a 0.8% gel electrophoresis.
 +
<BR><BR><BR>
'''General'''
'''General'''

Revision as of 14:24, 3 October 2013

Taxi.Coli: Smart Drug Delivery iGEM EPFL

Header

Cell Surface Display

PCR optimisation
To improve the pINP_construct amplification we did a gradient PCR at 72°C, 73°C and 74°C with 3% and 5% DMSO added to the mix. we verified our results with a 0.8% gel electrophoresis.


General

Competent cells try 3: it worked!