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Cell Surface DisplayPCR optimisation: to improve the pINP_construct amplification we did a gradient PCR at 72, 73 and 74°C with 3% and 5% DMSO added to the mix. we verified our results with a 0.8% gel electrophoresis.
PCR optimisation: to improve the pINP_construct amplification we did a gradient PCR at 72, 73 and 74°C with 3% and 5% DMSO added to the mix. we verified our results with a 0.8% gel electrophoresis.
