Team:EPF Lausanne/Calendar/29 August 2013

From 2013.igem.org

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Sensing <BR>
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<font size = "4"> Sensing </font> <BR>
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'''Results of GFP expression''' <BR>
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The inoculated bacteria did not grow at all in the arabinose containing medium. We thus innoculated three cultures but let them grow in a LB medium containing only chloramphenicol. We wanted to add the Arabinose once the Bacteria had already grown the next day.
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''Results of GFP expression'' <BR>
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-The inoculated bacteria did not grow at all in the arabinose containing medium. We thus innoculated three cultures but let them grow in a LB medium containing only chloramphenicol. We wanted to add the Arabinose once the Bacteria had already grown the next day.
'''Results of resuspension of K-12, MG 1655 e.coli''' <BR>
'''Results of resuspension of K-12, MG 1655 e.coli''' <BR>
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The LB was cloudy, indicating that the Bacteria had grown. We used 2ml to isolate the genomic DNA and the remaining 4ml were used to make glycerol stocks.
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-The LB was cloudy, indicating that the Bacteria had grown. We used 2ml to isolate the genomic DNA and the remaining 4ml were used to make glycerol stocks.
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<BR> <BR>
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<font size = "4"> Nanoparticles</font> <BR>
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'''Nanoparticles'''
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''Digestion of the Nanoparticles'' <BR>
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Again we tried to digest the nanoparticles with matrix metalloprotease MMP2 and again the nanoparticles are again a little bit smaller with MMP2.
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2 more measurements done : the nanoparticles are again a little bit smaller with MMP2.
 
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Revision as of 16:08, 3 October 2013

Taxi.Coli: Smart Drug Delivery iGEM EPFL

Header

Sensing

Results of GFP expression
-The inoculated bacteria did not grow at all in the arabinose containing medium. We thus innoculated three cultures but let them grow in a LB medium containing only chloramphenicol. We wanted to add the Arabinose once the Bacteria had already grown the next day.

Results of resuspension of K-12, MG 1655 e.coli
-The LB was cloudy, indicating that the Bacteria had grown. We used 2ml to isolate the genomic DNA and the remaining 4ml were used to make glycerol stocks.

Nanoparticles

Digestion of the Nanoparticles
Again we tried to digest the nanoparticles with matrix metalloprotease MMP2 and again the nanoparticles are again a little bit smaller with MMP2.


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