Sophierivara (Talk | contribs) (Created page with "{{Template:EPFL2013Header}} {{Template:EPFL2013Footer}}") |
Sandelisa90 (Talk | contribs) |
||
| Line 1: | Line 1: | ||
{{Template:EPFL2013Header}} | {{Template:EPFL2013Header}} | ||
| + | '''Cell Surface Display''' | ||
| + | ''Streptavidin:'' inoculation of the pET21a Streptavidin Dead we received in 3ml LB + Amp, 8hrs to make a glycerol Stock. | ||
| + | <br> We did the ''final PCR'' of ''pINP_construct'' with the following optimum condiitons: volume 50µl, 76°C, 5% DMSO. | ||
| + | <br> we did again a 0.8% electrophoresis gel to verify the PCR product. | ||
{{Template:EPFL2013Footer}} | {{Template:EPFL2013Footer}} | ||
Streptavidin: inoculation of the pET21a Streptavidin Dead we received in 3ml LB + Amp, 8hrs to make a glycerol Stock.
We did the final PCR of pINP_construct with the following optimum condiitons: volume 50µl, 76°C, 5% DMSO.
we did again a 0.8% electrophoresis gel to verify the PCR product.
"
