Sandelisa90 (Talk | contribs) |
GigiGoesGugu (Talk | contribs) |
||
| Line 1: | Line 1: | ||
{{Template:EPFL2013Header}} | {{Template:EPFL2013Header}} | ||
| - | '''Cell Surface Display''' | + | '''Cell Surface Display''' <BR> |
| + | |||
| + | ''Streptavidin'' <BR> | ||
| + | Inoculation of the pET21a Streptavidin Dead we received in 3ml LB + Amp, we incubated them for 8h to make a glycerol Stock. <BR> | ||
| + | Inoculation of the pET21a Streptavidin Dead we received in 3ml LB + Amp, overnight (16hrs) to make a miniprep the day after. | ||
| + | <br> We did the ''final PCR'' of ''pINP_construct'' with the following optimal condititons: volume 50µl, 76°C, 5% DMSO. | ||
| + | <br> We did a 0.8% electrophoresis gel to verify the PCR product. | ||
| - | |||
| - | |||
| - | |||
| - | |||
{{Template:EPFL2013Footer}} | {{Template:EPFL2013Footer}} | ||
Streptavidin
Inoculation of the pET21a Streptavidin Dead we received in 3ml LB + Amp, we incubated them for 8h to make a glycerol Stock.
Inoculation of the pET21a Streptavidin Dead we received in 3ml LB + Amp, overnight (16hrs) to make a miniprep the day after.
We did the final PCR of pINP_construct with the following optimal condititons: volume 50µl, 76°C, 5% DMSO.
We did a 0.8% electrophoresis gel to verify the PCR product.
"
