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Streptavidin: inoculation of the pET21a Streptavidin Dead we received in 3ml LB + Amp, 8hrs to make a glycerol Stock.
We did the final PCR of pINP_construct with the following optimum condiitons: volume 50µl, 76°C, 5% DMSO.
We did again a 0.8% electrophoresis gel to verify the PCR product.
Inoculation of the pET21a Streptavidin Dead we received in 3ml LB + Amp, overnight (16hrs) to make a miniprep the day after.