Team:EPF Lausanne/Calendar/31 July 2013


Revision as of 15:07, 1 October 2013 by Sandelisa90 (Talk | contribs)

Taxi.Coli: Smart Drug Delivery iGEM EPFL


Cell Surface Display Streptavidin Dead Cloning: Miniprep of pET21a Streptavidin Dead cells inoculated the day before.
PCR of pET21a Streptavidn Dead to amplify the Streptavidin sequence. The reaction was at 66°C, 68°C and 70°C without DMSO.
We did a 0.8% electrophoresis gel to verify the reaction.
As the gel result was good we did a larger amount (50µl) PCR at 68°C.
We did another Gel to check the product.
We did a PCR purification (Qiagen protocol) of the streptavidin dead obtained. We measured the Concentration at the nanodrop.