Team:EPF Lausanne/Calendar/3 September 2013

From 2013.igem.org

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<font size = "4"> Cell Surface Display</font> <BR>
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''Repeating Streptavidin Alive (SA) Gibson''<br>
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- DpnI digestion of the SA purifies PCR product to remove parental plasmid carry over.
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<br>- Gibson Reaction between the SA DpnI digested product (insert) and the INP_construct DpnI digested (backbone) in july.
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<br>- 0.8% electrophoresis gel to verify gibson product.
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<br>- DH5alpha competent cells transformation with Gibson product with negative controls transformations (PCR backbone, PCR insert and water).
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<br><br>
<font size = "4"> Sensing-Effector </font> <BR>
<font size = "4"> Sensing-Effector </font> <BR>

Revision as of 10:10, 4 October 2013

Taxi.Coli: Smart Drug Delivery iGEM EPFL

Header

Cell Surface Display
Repeating Streptavidin Alive (SA) Gibson
- DpnI digestion of the SA purifies PCR product to remove parental plasmid carry over.
- Gibson Reaction between the SA DpnI digested product (insert) and the INP_construct DpnI digested (backbone) in july.
- 0.8% electrophoresis gel to verify gibson product.
- DH5alpha competent cells transformation with Gibson product with negative controls transformations (PCR backbone, PCR insert and water).

Sensing-Effector

Gradient PCR of sensning constructs
-We used the new primers to run a PCR on the backbones and insert of the sensning constructs. But it did not work. Because of that we decided to do a gradient PCR.

Nanoparticles

Labelling of Nanoparticles
-We labelled the biotinylated gelatin nanoparticles with NHS-ester Cy5 dye.