Team:EPF Lausanne/Calendar/6 September 2013

From 2013.igem.org

(Difference between revisions)
Line 1: Line 1:
{{Template:EPFL2013Header}}
{{Template:EPFL2013Header}}
-
<font size = "4"> Sensing </font> <BR>
+
<font size = "4"> Sensing-Effector </font> <BR>
''PCR of the effector Backbones and the effector insert MMP2 and MMP9 without GFP'' <BR>
''PCR of the effector Backbones and the effector insert MMP2 and MMP9 without GFP'' <BR>

Revision as of 19:09, 3 October 2013

Taxi.Coli: Smart Drug Delivery iGEM EPFL

Header

Sensing-Effector

PCR of the effector Backbones and the effector insert MMP2 and MMP9 without GFP
-I did a 50ul PCR reaction of the Backbone and insert of the effector constructs. I again used a program where the first ten cylces are 10C° bellow the norma annealing temperature. The Gel electrophoresis showed that there was some sort of contamination, proably in the Primer Mixes. So I did the primer mixes again.

Nanoparticles

ELISE-like Assay
We finished the ELISA-like assay and it worked!