Team:EPF Lausanne/Protocols

From 2013.igem.org

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[https://static.igem.org/mediawiki/2013/b/bd/Team-EPF-Lausanne_Biotin_Staining_of_Streptavidin.pdf Biotin Staining of Streptavidin]<BR>
[https://static.igem.org/mediawiki/2013/b/bd/Team-EPF-Lausanne_Biotin_Staining_of_Streptavidin.pdf Biotin Staining of Streptavidin]<BR>
[https://static.igem.org/mediawiki/2013/a/a4/Team-EPF-Lausanne_Staining_of_Cell_Surface_Protein.pdf Direct Staining of cell surface Proteins] <BR>
[https://static.igem.org/mediawiki/2013/a/a4/Team-EPF-Lausanne_Staining_of_Cell_Surface_Protein.pdf Direct Staining of cell surface Proteins] <BR>
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[https://static.igem.org/mediawiki/2013/6/61/Team-EPF-Lausanne_Immunofl_staining_of_cellsurface_proteins.pdf Immunifluorescent staining of Cell Surface Proteins]
===Datasheets===
===Datasheets===

Revision as of 17:42, 4 October 2013

Taxi.Coli: Smart Drug Delivery iGEM EPFL

Header

Protocols

PCR
OD Measurements
PlateReader Measurements
Transforming Cells
Dialysis
ELISA-like Assay
Making Nanoparticles
Making Competent Cells
Gibson Assembly
Western Blot
Biotin Staining of Streptavidin
Direct Staining of cell surface Proteins
Immunifluorescent staining of Cell Surface Proteins

Datasheets

Below are listed the protocols we took directly from the datasheets/manufacturer instructions:

Biotinylation

Chemically attaching streptavidin to bacterial surface (using bacteria's outer glycoproteins)

Wiki Links

Retrieved from "http://2013.igem.org/Team:EPF_Lausanne/Protocols"