Team:Evry/Protocols/03

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<h1 align='center'> Protocole for Plasmid purification </h1>
<h1 align='center'> Protocole for Plasmid purification </h1>
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<p>For 100 µL of chemical competent cells, add 1 µL of plasmidic DNA.<br/>
 
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Let 30 minutes on ice.<br/>
 
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Let the cells at 42°C for exactly 50 seconds.<br/>
 
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Let 5 minutes on ice.<br/>
 
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Resuspend the cells with 1 mL of LB spread on a petri dish then let it at 37°C.<br/>
 
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<br/></p>
 
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Revision as of 13:14, 29 July 2013

Protocole for Plasmid purification

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