Team:Exeter/Parts

From 2013.igem.org

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An important aspect of the iGEM competition is the use and creation of standard  biological parts. Each team will make new parts during iGEM and will place them in the [http://partsregistry.org Registry of Standard Biological Parts]. The iGEM software provides an easy way to present the parts your team has created . The "groupparts" tag will generate a table with all of the parts that your team adds to your team sandbox.  Note that if you want to document a part you need to document it on the [http://partsregistry.org Registry], not on your team wiki.
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== Parts ==
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Remember that the goal of proper part documentation is to describe and define a part such that it can be used without a need to refer to the primary literature. The next iGEM team should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for  users who wish to know more.
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''This page will document the parts that Exeter iGEM 2013 submit to the biobrick registry.''
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<groupparts>iGEM013 Exeter</groupparts>
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Our aim is to submit a composite biobrick for <i>E. coli</i> which will allow control three genes of your choice using red, green and blue ([http://en.wikipedia.org/wiki/RGB_color_model RGB]) light. We have successful sent in the composite part for the green light module ([http://parts.igem.org/wiki/index.php?title=Part:BBa_K1173501]). We also aim to create constituent bio-bricks for red/blue/green light input matching cyan/yellow/magenta pigments respectively. We have managed to submit a brick for the red light module (includes the cyan pigment, see [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1173500]). We will also submit a constituent bio-brick for red light input with cut sites to allow input of your favourite gene ([http://parts.igem.org/wiki/index.php?title=Part:BBa_K1173502]). See our [[Team:Exeter/Project|project section]] for details about our bio-bricks.
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We plan to extensively characterise our system and provide a mathematical model of our system for quantative control of outputs. See our [[Team:Exeter/Modelling|modelling section]].
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{| style="color:#2F2F4F;background-color:#B9D3EE;" cellpadding="3" cellspacing="1" align="center"
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!align="center"|[[Team:Exeter|Home]]
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'''Table including all of the parts we are hoping to utilise'''
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!align="center"|[[Team:Exeter/Team|Team]]
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!align="center"|[https://igem.org/Team.cgi?year=2013&team_name=Exeter Official Team Profile]
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{| class="wikitable"
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!align="center"|[[Team:Exeter/Project|Project]]
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|-
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!align="center"|[[Team:Exeter/Parts|Parts Submitted to the Registry]]
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! Part Code and link to Registry page !! Name of Part
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!align="center"|[[Team:Exeter/Modeling|Modeling]]
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|-
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!align="center"|[[Team:Exeter/Notebook|Notebook]]
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|[http://parts.igem.org/wiki/index.php?title=Part:BBa_K608002 BBa_K608002] || Strong promoter and strong RBS
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!align="center"|[[Team:Exeter/Safety|Safety]]
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|-
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!align="center"|[[Team:Exeter/Attributions|Attributions]]
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| [http://parts.igem.org/wiki/index.php?title=Part:BBa_I712074 BBa_I712074] || T7 promoter
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| [http://parts.igem.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015] || Terminator
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| [http://parts.igem.org/wiki/index.php?title=Part:BBa_Q04510 BBa_Q04510] || lambda cI inverter system
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| [http://parts.igem.org/wiki/index.php?title=Part:BBa_K322124 BBa_K322124] || Cph8 red light sensor with RBS, version one
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| [http://parts.igem.org/wiki/index.php?title=Part:BBa_K592018 BBa_K592018]|| Cph8 red light sensor with RBS, version two
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| [http://parts.igem.org/wiki/index.php?title=Part:BBa_K098011 BBa_K098011] || OmpR, the protein which communicates between the red light sensor and the cyan pigment gene
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| [http://parts.igem.org/wiki/index.php?title=Part:BBa_R0082 BBa_R0082] || OmpR specific promoter, only allows transcription when OmpR-P is present
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| [http://parts.igem.org/wiki/index.php?title=Part:BBa_K322122 BBa_K322122] || Cyan pigment, version one (composed of HO1 and pcyA, makes phycocyanobillin)
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| [http://parts.igem.org/wiki/index.php?title=Part:BBa_K909000 BBa_K909000] || Cyan pigment, version one, includes stronger RBS than BBa_K322122
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| [http://parts.igem.org/wiki/index.php?title=Part:BBa_K592011 BBa_K592011] || Cyan pigment, version two
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| [http://parts.igem.org/wiki/index.php?title=Part:BBa_K864404 BBa_K864404] || Cyan pigment, version two, includes a promoter and RBS
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| [http://parts.igem.org/wiki/index.php?title=Part:BBa_K592022 BBa_K592022] || Cyan pigment, version two, includes a alternative promoter and RBS
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| [http://parts.igem.org/wiki/index.php?title=Part:BBa_K592004 BBa_K592004] || YF1 blue light sensor
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| [http://parts.igem.org/wiki/index.php?title=Part:BBa_K592005 BBa_K592005] || FixJ, the protein which communicates between the blue light sensor and the yellow pigment gene
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| [http://parts.igem.org/wiki/index.php?title=Part:BBa_K592006 BBa_K592006] || FixJ specific promoter, only allows transcription when FixJ-P is present
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| [http://parts.igem.org/wiki/index.php?title=Part:BBa_K592010 BBa_K592010] || Yellow pigment, version one
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| [http://parts.igem.org/wiki/index.php?title=Part:BBa_S05058 BBa_S05058] || Yellow pigment, version one, with added RBS
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| [http://parts.igem.org/wiki/index.php?title=Part:BBa_K592001 BBa_K592001] || CcaS green light sensor
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| [http://parts.igem.org/wiki/index.php?title=Part:BBa_K592002 BBa_K592002] || CcaR, the protein which communicates between the green light sensor and the lambda inverter system
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|-
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| [http://parts.igem.org/wiki/index.php?title=Part:BBa_K592017 BBa_K592017] || Combined CcaS and CcaR with RBS before each
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| [http://parts.igem.org/wiki/index.php?title=Part:BBa_K592003 BBa_K592003] || CcaR specific promoter, only allows transcription when CcaR-P is '''NOT''' present
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| [http://parts.igem.org/wiki/index.php?title=Part:BBa_K592012 BBa_K592012] || Magenta pigment
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| [http://parts.igem.org/wiki/index.php?title=Part:BBa_J04450 BBa_KJ04450] || RFP control
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| [http://parts.igem.org/wiki/index.php?title=Part:BBa_I746915 BBa_I746915] || Superfolder GFP
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[[File:John's plasmid designs.png|left||900px|Image: 900 pixels]]
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<groupparts>iGEM013 Exeter</groupparts>
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        </div>
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  </div>
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{{:Team:Exeter/Template/Footer}}

Latest revision as of 00:23, 5 October 2013

Exeter iGEM 2013 · Paint by Coli

Parts

This page will document the parts that Exeter iGEM 2013 submit to the biobrick registry.

Our aim is to submit a composite biobrick for E. coli which will allow control three genes of your choice using red, green and blue (RGB) light. We have successful sent in the composite part for the green light module ([1]). We also aim to create constituent bio-bricks for red/blue/green light input matching cyan/yellow/magenta pigments respectively. We have managed to submit a brick for the red light module (includes the cyan pigment, see [2]). We will also submit a constituent bio-brick for red light input with cut sites to allow input of your favourite gene ([3]). See our project section for details about our bio-bricks.

We plan to extensively characterise our system and provide a mathematical model of our system for quantative control of outputs. See our modelling section.

Table including all of the parts we are hoping to utilise

Part Code and link to Registry page Name of Part
BBa_K608002 Strong promoter and strong RBS
BBa_I712074 T7 promoter
BBa_B0015 Terminator
BBa_Q04510 lambda cI inverter system
BBa_K322124 Cph8 red light sensor with RBS, version one
BBa_K592018 Cph8 red light sensor with RBS, version two
BBa_K098011 OmpR, the protein which communicates between the red light sensor and the cyan pigment gene
BBa_R0082 OmpR specific promoter, only allows transcription when OmpR-P is present
BBa_K322122 Cyan pigment, version one (composed of HO1 and pcyA, makes phycocyanobillin)
BBa_K909000 Cyan pigment, version one, includes stronger RBS than BBa_K322122
BBa_K592011 Cyan pigment, version two
BBa_K864404 Cyan pigment, version two, includes a promoter and RBS
BBa_K592022 Cyan pigment, version two, includes a alternative promoter and RBS
BBa_K592004 YF1 blue light sensor
BBa_K592005 FixJ, the protein which communicates between the blue light sensor and the yellow pigment gene
BBa_K592006 FixJ specific promoter, only allows transcription when FixJ-P is present
BBa_K592010 Yellow pigment, version one
BBa_S05058 Yellow pigment, version one, with added RBS
BBa_K592001 CcaS green light sensor
BBa_K592002 CcaR, the protein which communicates between the green light sensor and the lambda inverter system
BBa_K592017 Combined CcaS and CcaR with RBS before each
BBa_K592003 CcaR specific promoter, only allows transcription when CcaR-P is NOT present
BBa_K592012 Magenta pigment
BBa_KJ04450 RFP control
BBa_I746915 Superfolder GFP
Image: 900 pixels

<groupparts>iGEM013 Exeter</groupparts>

Exeter iGEM 2013 · Paint by Coli

Retrieved from "http://2013.igem.org/Team:Exeter/Parts"