Team:Exeter/Parts
From 2013.igem.org
Fentwistle (Talk | contribs) |
|||
(23 intermediate revisions not shown) | |||
Line 1: | Line 1: | ||
- | + | {{:Team:Exeter/Template/Header}} | |
- | { | + | <div class="container"> |
- | + | <div class="row"> | |
- | + | <div class="span" style="text-align:justify"> | |
- | + | == Parts == | |
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
''This page will document the parts that Exeter iGEM 2013 submit to the biobrick registry.'' | ''This page will document the parts that Exeter iGEM 2013 submit to the biobrick registry.'' | ||
- | Our aim is to submit a composite biobrick for E. coli which will allow control three genes of your choice using | + | Our aim is to submit a composite biobrick for <i>E. coli</i> which will allow control three genes of your choice using red, green and blue ([http://en.wikipedia.org/wiki/RGB_color_model RGB]) light. We have successful sent in the composite part for the green light module ([http://parts.igem.org/wiki/index.php?title=Part:BBa_K1173501]). We also aim to create constituent bio-bricks for red/blue/green light input matching cyan/yellow/magenta pigments respectively. We have managed to submit a brick for the red light module (includes the cyan pigment, see [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1173500]). We will also submit a constituent bio-brick for red light input with cut sites to allow input of your favourite gene ([http://parts.igem.org/wiki/index.php?title=Part:BBa_K1173502]). See our [[Team:Exeter/Project|project section]] for details about our bio-bricks. |
We plan to extensively characterise our system and provide a mathematical model of our system for quantative control of outputs. See our [[Team:Exeter/Modelling|modelling section]]. | We plan to extensively characterise our system and provide a mathematical model of our system for quantative control of outputs. See our [[Team:Exeter/Modelling|modelling section]]. | ||
Line 23: | Line 17: | ||
! Part Code and link to Registry page !! Name of Part | ! Part Code and link to Registry page !! Name of Part | ||
|- | |- | ||
- | | [http://parts.igem.org/wiki/index.php?title=Part:BBa_K608002 BBa_K608002] || Strong promoter and strong RBS | + | |[http://parts.igem.org/wiki/index.php?title=Part:BBa_K608002 BBa_K608002] || Strong promoter and strong RBS |
|- | |- | ||
| [http://parts.igem.org/wiki/index.php?title=Part:BBa_I712074 BBa_I712074] || T7 promoter | | [http://parts.igem.org/wiki/index.php?title=Part:BBa_I712074 BBa_I712074] || T7 promoter | ||
Line 40: | Line 34: | ||
|- | |- | ||
| [http://parts.igem.org/wiki/index.php?title=Part:BBa_K322122 BBa_K322122] || Cyan pigment, version one (composed of HO1 and pcyA, makes phycocyanobillin) | | [http://parts.igem.org/wiki/index.php?title=Part:BBa_K322122 BBa_K322122] || Cyan pigment, version one (composed of HO1 and pcyA, makes phycocyanobillin) | ||
+ | |- | ||
+ | | [http://parts.igem.org/wiki/index.php?title=Part:BBa_K909000 BBa_K909000] || Cyan pigment, version one, includes stronger RBS than BBa_K322122 | ||
|- | |- | ||
| [http://parts.igem.org/wiki/index.php?title=Part:BBa_K592011 BBa_K592011] || Cyan pigment, version two | | [http://parts.igem.org/wiki/index.php?title=Part:BBa_K592011 BBa_K592011] || Cyan pigment, version two | ||
Line 45: | Line 41: | ||
| [http://parts.igem.org/wiki/index.php?title=Part:BBa_K864404 BBa_K864404] || Cyan pigment, version two, includes a promoter and RBS | | [http://parts.igem.org/wiki/index.php?title=Part:BBa_K864404 BBa_K864404] || Cyan pigment, version two, includes a promoter and RBS | ||
|- | |- | ||
- | | [http://parts.igem.org/wiki/index.php?title=Part:BBa_K592022 | + | | [http://parts.igem.org/wiki/index.php?title=Part:BBa_K592022 BBa_K592022] || Cyan pigment, version two, includes a alternative promoter and RBS |
- | + | ||
- | + | ||
|- | |- | ||
| [http://parts.igem.org/wiki/index.php?title=Part:BBa_K592004 BBa_K592004] || YF1 blue light sensor | | [http://parts.igem.org/wiki/index.php?title=Part:BBa_K592004 BBa_K592004] || YF1 blue light sensor | ||
Line 66: | Line 60: | ||
|- | |- | ||
| [http://parts.igem.org/wiki/index.php?title=Part:BBa_K592003 BBa_K592003] || CcaR specific promoter, only allows transcription when CcaR-P is '''NOT''' present | | [http://parts.igem.org/wiki/index.php?title=Part:BBa_K592003 BBa_K592003] || CcaR specific promoter, only allows transcription when CcaR-P is '''NOT''' present | ||
- | | | + | |- |
| [http://parts.igem.org/wiki/index.php?title=Part:BBa_K592012 BBa_K592012] || Magenta pigment | | [http://parts.igem.org/wiki/index.php?title=Part:BBa_K592012 BBa_K592012] || Magenta pigment | ||
+ | |- | ||
+ | | [http://parts.igem.org/wiki/index.php?title=Part:BBa_J04450 BBa_KJ04450] || RFP control | ||
+ | |- | ||
+ | | [http://parts.igem.org/wiki/index.php?title=Part:BBa_I746915 BBa_I746915] || Superfolder GFP | ||
|} | |} | ||
+ | [[File:John's plasmid designs.png|left||900px|Image: 900 pixels]] | ||
<groupparts>iGEM013 Exeter</groupparts> | <groupparts>iGEM013 Exeter</groupparts> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | {{:Team:Exeter/Template/Footer}} |
Latest revision as of 00:23, 5 October 2013
Parts
This page will document the parts that Exeter iGEM 2013 submit to the biobrick registry.
Our aim is to submit a composite biobrick for E. coli which will allow control three genes of your choice using red, green and blue (RGB) light. We have successful sent in the composite part for the green light module ([1]). We also aim to create constituent bio-bricks for red/blue/green light input matching cyan/yellow/magenta pigments respectively. We have managed to submit a brick for the red light module (includes the cyan pigment, see [2]). We will also submit a constituent bio-brick for red light input with cut sites to allow input of your favourite gene ([3]). See our project section for details about our bio-bricks.
We plan to extensively characterise our system and provide a mathematical model of our system for quantative control of outputs. See our modelling section.
Table including all of the parts we are hoping to utilise
Part Code and link to Registry page | Name of Part |
---|---|
BBa_K608002 | Strong promoter and strong RBS |
BBa_I712074 | T7 promoter |
BBa_B0015 | Terminator |
BBa_Q04510 | lambda cI inverter system |
BBa_K322124 | Cph8 red light sensor with RBS, version one |
BBa_K592018 | Cph8 red light sensor with RBS, version two |
BBa_K098011 | OmpR, the protein which communicates between the red light sensor and the cyan pigment gene |
BBa_R0082 | OmpR specific promoter, only allows transcription when OmpR-P is present |
BBa_K322122 | Cyan pigment, version one (composed of HO1 and pcyA, makes phycocyanobillin) |
BBa_K909000 | Cyan pigment, version one, includes stronger RBS than BBa_K322122 |
BBa_K592011 | Cyan pigment, version two |
BBa_K864404 | Cyan pigment, version two, includes a promoter and RBS |
BBa_K592022 | Cyan pigment, version two, includes a alternative promoter and RBS |
BBa_K592004 | YF1 blue light sensor |
BBa_K592005 | FixJ, the protein which communicates between the blue light sensor and the yellow pigment gene |
BBa_K592006 | FixJ specific promoter, only allows transcription when FixJ-P is present |
BBa_K592010 | Yellow pigment, version one |
BBa_S05058 | Yellow pigment, version one, with added RBS |
BBa_K592001 | CcaS green light sensor |
BBa_K592002 | CcaR, the protein which communicates between the green light sensor and the lambda inverter system |
BBa_K592017 | Combined CcaS and CcaR with RBS before each |
BBa_K592003 | CcaR specific promoter, only allows transcription when CcaR-P is NOT present |
BBa_K592012 | Magenta pigment |
BBa_KJ04450 | RFP control |
BBa_I746915 | Superfolder GFP |
<groupparts>iGEM013 Exeter</groupparts>