Team:Frankfurt/Project/Results

From 2013.igem.org

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=DNA constructs for realisation of our idea =
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== DNA constructs for realisation of our idea == __NOEDITSECTION__
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=Gap repair Cloning for Vector Assembly=
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== Gap repair Cloning for Vector Assembly == __NOEDITSECTION__
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Gap repair means the homologue recombination in yeast in order to assemble desired DNA fragments. It is easier and more effective than cloning via restriction and ligation. This method was used to assemble the mevalonate overexpression plasmid in year 2012. Since in last year the KAH fragment, which is crutial for the second plasmid, was not able to be constructed the reconstruction of the steviol plasmid via gap repair was done this year. We were able to grow cultures containig the plasmid on selective media with the corresponding auxotrphy, but unfortunately we were to short on time to
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extract it.
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= Gas Chromatographic Analysis =
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== Gas Chromatographic Analysis == __NOEDITSECTION__
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Revision as of 01:19, 5 October 2013

Team: iGEM Frankfurt - 2012.igem.org

DNA constructs for realisation of our idea

Gap repair Cloning for Vector Assembly

Gap repair means the homologue recombination in yeast in order to assemble desired DNA fragments. It is easier and more effective than cloning via restriction and ligation. This method was used to assemble the mevalonate overexpression plasmid in year 2012. Since in last year the KAH fragment, which is crutial for the second plasmid, was not able to be constructed the reconstruction of the steviol plasmid via gap repair was done this year. We were able to grow cultures containig the plasmid on selective media with the corresponding auxotrphy, but unfortunately we were to short on time to extract it.

Gas Chromatographic Analysis

iGEM-Team Frankfurt 2013