Team:Groningen/Labwork/17 August 2013

From 2013.igem.org

(Difference between revisions)
(Created page with "<html> <head> <meta http-equiv="X-UA-Compatible" content="IE=EDGE" charset="utf-8" /> <link rel="stylesheet" href="https://2013.igem.org/Team:Groningen/CSS/DefaultPage?action=ra...")
Line 24: Line 24:
<h2>Inne</h2>
<h2>Inne</h2>
For the plates of the motility assay only the 0.4% provided a probable result. The others were completely overgrown, the reason for this is very likely that the concentration of agar (0.2% and 0.1%) is to low for plates. The tests did show that delta cheY bacillus grows better, and more uniformly, then competent E.coli.
For the plates of the motility assay only the 0.4% provided a probable result. The others were completely overgrown, the reason for this is very likely that the concentration of agar (0.2% and 0.1%) is to low for plates. The tests did show that delta cheY bacillus grows better, and more uniformly, then competent E.coli.
-
 
+
<br>
-
The hy-spank GFP e.coli plates grew colonies, 4 single colonies will be picked and inoculated.
+
<br>The hy-spank GFP e.coli plates grew colonies, 4 single colonies will be picked and inoculated.
-
 
+
<br>
-
Performed a restriction digest of ms2 samples 1 till 3.
+
<br>Performed a restriction digest of ms2 samples 1 till 3.
-
 
+
<br> 0.8% gel was loaded as following.
 +
<table border = "1">
 +
<tr>
 +
<td>slot 1</td>
 +
<td>slot 2</td>
 +
<td>slot 3</td>
 +
<td>slot 4</td>
 +
<td>slot 5</td>
 +
<td>slot 6</td>
 +
<td>slot 7</td>
 +
<td>slot 8</td>
 +
<td>slot 9</td>
 +
<td>slot 10</td>
 +
</tr>
 +
<tr>
 +
<td>1kb DNA ruler</td>
 +
<td>ms2 1</td>
 +
<td>ms2 2</td>
 +
<td>ms2 3</td>
 +
<td>uncut plasmid ms2 1</td>
 +
<td>1kb DNA ruler</td>
 +
<td>ms2 1</td>
 +
<td>ms2 2</td>
 +
<td>ms2 3</td>
 +
<td>uncut plasmid ms2 1</td>
 +
</tr>
 +
</table>
   
   
</div>
</div>

Revision as of 14:55, 17 August 2013

Inne

For the plates of the motility assay only the 0.4% provided a probable result. The others were completely overgrown, the reason for this is very likely that the concentration of agar (0.2% and 0.1%) is to low for plates. The tests did show that delta cheY bacillus grows better, and more uniformly, then competent E.coli.

The hy-spank GFP e.coli plates grew colonies, 4 single colonies will be picked and inoculated.

Performed a restriction digest of ms2 samples 1 till 3.
0.8% gel was loaded as following.
slot 1 slot 2 slot 3 slot 4 slot 5 slot 6 slot 7 slot 8 slot 9 slot 10
1kb DNA ruler ms2 1 ms2 2 ms2 3 uncut plasmid ms2 1 1kb DNA ruler ms2 1 ms2 2 ms2 3 uncut plasmid ms2 1