Team:Groningen/Labwork/17 August 2013
From 2013.igem.org
(Difference between revisions)
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For the plates of the motility assay only the 0.4% provided a probable result. The others were completely overgrown, the reason for this is very likely that the concentration of agar (0.2% and 0.1%) is to low for plates. The tests did show that delta cheY bacillus grows better, and more uniformly, then competent E.coli. | For the plates of the motility assay only the 0.4% provided a probable result. The others were completely overgrown, the reason for this is very likely that the concentration of agar (0.2% and 0.1%) is to low for plates. The tests did show that delta cheY bacillus grows better, and more uniformly, then competent E.coli. | ||
<br> | <br> | ||
- | <br>The hy-spank GFP e.coli plates grew colonies, 4 single colonies | + | <br>The hy-spank GFP e.coli plates grew colonies, 4 single colonies are picked and inoculated in the 37C shaker (overnight). |
<br> | <br> | ||
<br>Performed a restriction digest of ms2 samples 1 till 3. | <br>Performed a restriction digest of ms2 samples 1 till 3. | ||
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The gel was loaded in duplo because there was to much sample to fit in one well | The gel was loaded in duplo because there was to much sample to fit in one well | ||
+ | <br>the gel didn't show any bands except for the DNA ruler. | ||
</div> | </div> | ||
Latest revision as of 15:25, 17 August 2013
Inne
For the plates of the motility assay only the 0.4% provided a probable result. The others were completely overgrown, the reason for this is very likely that the concentration of agar (0.2% and 0.1%) is to low for plates. The tests did show that delta cheY bacillus grows better, and more uniformly, then competent E.coli.The hy-spank GFP e.coli plates grew colonies, 4 single colonies are picked and inoculated in the 37C shaker (overnight).
Performed a restriction digest of ms2 samples 1 till 3.
0.8% gel was loaded as following.
well 1 | well 2 | well 3 | well 4 | well 5 | well 6 | well 7 | well 8 | well 9 | well 10 |
1kb DNA ruler | ms2 1 | ms2 2 | ms2 3 | uncut plasmid ms2 1 | 1kb DNA ruler | ms2 1 | ms2 2 | ms2 3 | uncut plasmid ms2 1 |
the gel didn't show any bands except for the DNA ruler.