Team:Groningen/Labwork/17 August 2013

From 2013.igem.org

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For the plates of the motility assay only the 0.4% provided a probable result. The others were completely overgrown, the reason for this is very likely that the concentration of agar (0.2% and 0.1%) is to low for plates. The tests did show that delta cheY bacillus grows better, and more uniformly, then competent E.coli.
For the plates of the motility assay only the 0.4% provided a probable result. The others were completely overgrown, the reason for this is very likely that the concentration of agar (0.2% and 0.1%) is to low for plates. The tests did show that delta cheY bacillus grows better, and more uniformly, then competent E.coli.
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<br>The hy-spank GFP e.coli plates grew colonies, 4 single colonies will be picked and inoculated.
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<br>The hy-spank GFP e.coli plates grew colonies, 4 single colonies are picked and inoculated in the 37C shaker (overnight).
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<br>Performed a restriction digest of ms2 samples 1 till 3.
<br>Performed a restriction digest of ms2 samples 1 till 3.
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The gel was loaded in duplo because there was to much sample to fit in one well
The gel was loaded in duplo because there was to much sample to fit in one well
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<br>the gel didn't show any bands except for the DNA ruler.
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Latest revision as of 15:25, 17 August 2013

Inne

For the plates of the motility assay only the 0.4% provided a probable result. The others were completely overgrown, the reason for this is very likely that the concentration of agar (0.2% and 0.1%) is to low for plates. The tests did show that delta cheY bacillus grows better, and more uniformly, then competent E.coli.

The hy-spank GFP e.coli plates grew colonies, 4 single colonies are picked and inoculated in the 37C shaker (overnight).

Performed a restriction digest of ms2 samples 1 till 3.
0.8% gel was loaded as following.
well 1 well 2 well 3 well 4 well 5 well 6 well 7 well 8 well 9 well 10
1kb DNA ruler ms2 1 ms2 2 ms2 3 uncut plasmid ms2 1 1kb DNA ruler ms2 1 ms2 2 ms2 3 uncut plasmid ms2 1
The gel was loaded in duplo because there was to much sample to fit in one well
the gel didn't show any bands except for the DNA ruler.