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Team:Groningen/Labwork/19 July 2013
From 2013.igem.org
(Difference between revisions)
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<h2>Sander and Erik Jan</h2> | <h2>Sander and Erik Jan</h2> | ||
| - | <br> Did a PCR of Silk 2 (strepF-R) and Silk 3 (F-StrepR). | + | <br> Did a <a href="https://2013.igem.org/Team:Groningen/protocols/PCR"><FONT COLOR="black"><b>PCR</b></FONT></a> of Silk 2 (strepF-R) and Silk 3 (F-StrepR). |
<br> annealing temperatures of 78 C for silk 2 an 80 C for silk 3 | <br> annealing temperatures of 78 C for silk 2 an 80 C for silk 3 | ||
<h2>Sander</h2> | <h2>Sander</h2> | ||
| - | <br> ran restriction analysis of silk 1 (F-R) on 0,8% agarose gel. 90 V for 34 min. | + | <br> ran restriction analysis of silk 1 (F-R) on 0,8% agarose <a href="https://2013.igem.org/Team:Groningen/protocols/GelElectrophoresis"><FONT COLOR="black"><b>gel</b></FONT></a>. 90 V for 34 min. |
<br> low concentration on gel. | <br> low concentration on gel. | ||
| - | <br> did a ligation reaction on silk 1 with each of the Signal Sequences (MotB, FliZ, EstA and LytB) at 1:1 ratio. | + | <br> did a <a href="https://2013.igem.org/Team:Groningen/protocols/Ligation"><FONT COLOR="black"><b>ligation reaction</b></FONT></a> on silk 1 with each of the Signal Sequences (MotB, FliZ, EstA and LytB) at 1:1 ratio. |
| - | <br> did a PCR of ligation reactions. same protocol as silk 3 above. | + | <br> did a <a href="https://2013.igem.org/Team:Groningen/protocols/PCR"><FONT COLOR="black"><b>PCR</b></FONT></a> of ligation reactions. same protocol as silk 3 above. |
<h2>Claudio and Mike</h2> | <h2>Claudio and Mike</h2> | ||
Latest revision as of 11:24, 30 July 2013
Inne
No colonies have grown on the cm plates.
There were supposed to be colonies that contained the GFP biobrick.
Sander and Erik Jan
Did a PCR of Silk 2 (strepF-R) and Silk 3 (F-StrepR).
annealing temperatures of 78 C for silk 2 an 80 C for silk 3
Sander
ran restriction analysis of silk 1 (F-R) on 0,8% agarose gel. 90 V for 34 min.
low concentration on gel.
did a ligation reaction on silk 1 with each of the Signal Sequences (MotB, FliZ, EstA and LytB) at 1:1 ratio.
did a PCR of ligation reactions. same protocol as silk 3 above.
Claudio and Mike
The final DNA sequences are checked and discussed with the one of the advisor (Ruud Detert Oude Weme).
The sequences are eventually ordered.
