Team:Groningen/Labwork/22 August 2013

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Revision as of 14:16, 22 August 2013

Claudio

S1, S2, S5 and S6 show colonies.
SS1 and SS2 do not show any colonies.

Negative and positive control show no colony and thousands of colonies, respectively.

ColonyPCR was performed using VF2 and VR. One colon per plate was screened.
The reaction was checked on agarose gel 0.8%.
The gel shows bands at the expected height.

Sebas

Transformation yesterday:
- negative control: 3 small colonies (somehow resistant to amp)
- 100µl transformation mix: ~50 small colonies
- On the extra concentrated plate (EC): ~300 small colonies + 11 big colonies

Restreaked 6 of the big colonies on plate and grew them in 3ml LB/amp.