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Team:Groningen/Labwork/25 June 2013
From 2013.igem.org
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<br>Both LB media are autoclaved together with tooth picks, 1.5 and 2 ml eppendorf tubes. After autoclaving, the LB medium with agar is placed in the 55°C stove. | <br>Both LB media are autoclaved together with tooth picks, 1.5 and 2 ml eppendorf tubes. After autoclaving, the LB medium with agar is placed in the 55°C stove. | ||
| + | <p> | ||
<h2>Mirjam and Claudio</h2> | <h2>Mirjam and Claudio</h2> | ||
| - | < | + | <i>B. subtilis</i> strain 168 is inoculated in LB medium O/N at 37°C and every buffer necessary for the extraction of genomic DNA is arranged. |
| + | <p> | ||
<h2>Claudio</h2> | <h2>Claudio</h2> | ||
Continued the design for the heat motility. | Continued the design for the heat motility. | ||
Revision as of 15:24, 28 July 2013
Mirjam, Chaline and Inne
A couple of media are prepared:-LB medium with agar 1 liter
-LB medium 0.5 liter divided into 60/80 ml portions
-70% ethanol
-1x TBE buffer 2 liter
Both LB media are autoclaved together with tooth picks, 1.5 and 2 ml eppendorf tubes. After autoclaving, the LB medium with agar is placed in the 55°C stove.
Mirjam and Claudio
B. subtilis strain 168 is inoculated in LB medium O/N at 37°C and every buffer necessary for the extraction of genomic DNA is arranged.
