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Team:Groningen/Labwork/3 July 2013
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<br/>Run a 0.8% agarose gel to examine the purified promoter, gel run at 100V for 22 minutes. | <br/>Run a 0.8% agarose gel to examine the purified promoter, gel run at 100V for 22 minutes. | ||
| - | + | <h2>Mirjam and Sander</h2> | |
<br/>The gel examined that again dimerization of the primers happened. Therefore a new PCR run is made with the addition of DMSO. | <br/>The gel examined that again dimerization of the primers happened. Therefore a new PCR run is made with the addition of DMSO. | ||
Turned out that the one with DMSO had better results. Did a gel purification (standard protocol). | Turned out that the one with DMSO had better results. Did a gel purification (standard protocol). | ||
| - | + | <h2>Sander and Inne</h2> | |
<br/>Did a PCR for the silk template, in duplo. | <br/>Did a PCR for the silk template, in duplo. | ||
With the following protocol: | With the following protocol: | ||
Revision as of 11:56, 24 July 2013
Mirjam
Run a 0.8% agarose gel to examine the purified promoter, gel run at 100V for 22 minutes.
Mirjam and Sander
The gel examined that again dimerization of the primers happened. Therefore a new PCR run is made with the addition of DMSO.
Turned out that the one with DMSO had better results. Did a gel purification (standard protocol).
Sander and Inne
Did a PCR for the silk template, in duplo.
With the following protocol:
1 2 3 4 5 6
98 98 85 72 72 4
2:00 0:30 0:25 0:27 10:00 forever
So two with DMSO and two without. NB: another change is that we used 5% DMSO this time
Ran a gel which had more bigger bands them the previous pcr. The samples without DMSO were clearer than those with DMSO.
