Team:Groningen/Labwork/3 July 2013

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<h2>Mirjam</h2>
<h2>Mirjam</h2>
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<br/>Run a 0.8% agarose gel to examine the purified promoter, gel run at 100V for 22 minutes.
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<br/>Run a 0.8% agarose <a href="https://2013.igem.org/Team:Groningen/protocols/GelElectrophoresis"><FONT COLOR="black"><b>gel</b></FONT></a> to examine the purified promoter, gel run at 100V for 22 minutes.
<h2>Mirjam and Sander</h2>
<h2>Mirjam and Sander</h2>
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<br/>The gel examined that again dimerization of the primers happened. Therefore a new PCR run is made with the addition of DMSO.
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<br/>The gel examined that again dimerization of the primers happened. Therefore a new <a href="https://2013.igem.org/Team:Groningen/protocols/PCR"><FONT COLOR="black"><b>PCR</b></FONT></a> run is made with the addition of DMSO.
Turned out that the one with DMSO had better results. Did a gel purification (standard protocol).
Turned out that the one with DMSO had better results. Did a gel purification (standard protocol).
<h2>Sander and Inne</h2>
<h2>Sander and Inne</h2>
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<br/>Did a PCR for the silk template, in duplo.
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<br/>Did a <a href="https://2013.igem.org/Team:Groningen/protocols/PCR"><FONT COLOR="black"><b>PCR</b></FONT></a> for the silk template, in duplo.
With the following protocol:
With the following protocol:
<br>1 2 3 4 5 6   
<br>1 2 3 4 5 6   
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NB: another change is that we used 5% DMSO this time
NB: another change is that we used 5% DMSO this time
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Ran a gel which had more bigger bands them the previous pcr.
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Ran a <a href="https://2013.igem.org/Team:Groningen/protocols/GelElectrophoresis"><FONT COLOR="black"><b>gel</b></FONT></a> which had more bigger bands them the previous pcr.
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The samples without DMSO were clearer than those with DMSO.
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The samples without DMSO were clearer than those with DMSO.  
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Latest revision as of 09:45, 30 July 2013

Mirjam


Run a 0.8% agarose gel to examine the purified promoter, gel run at 100V for 22 minutes.

Mirjam and Sander


The gel examined that again dimerization of the primers happened. Therefore a new PCR run is made with the addition of DMSO. Turned out that the one with DMSO had better results. Did a gel purification (standard protocol).

Sander and Inne


Did a PCR for the silk template, in duplo. With the following protocol:
1 2 3 4 5 6
98 98 85 72 72 4
2:00 0:30 0:25 0:27 10:00 forever So two with DMSO and two without. NB: another change is that we used 5% DMSO this time Ran a gel which had more bigger bands them the previous pcr. The samples without DMSO were clearer than those with DMSO.