Team:Heidelberg/Tyrocidine week23 variation
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===Restriction digest of LV3B,3C,3D,5A,5B and LV8A=== | ===Restriction digest of LV3B,3C,3D,5A,5B and LV8A=== | ||
Latest revision as of 17:20, 4 October 2013
Restriction digest of LV3B,3C,3D,5A,5B and LV8A
A miniprep of these constructs was done by the use of the QuiaPrep Kit. Measurements via nanodrop displayed the following concentrations:
Construct | Concentration [ng/µl] | A260/280 |
---|---|---|
LV3B | 240,5 | 4,809/2,51 |
LV3C | 170,3 | 3,41/1,81 |
LV3D | 197,8 | 3,96/2,07 |
LV5A | 212,6 | 4,25/1,256 |
LV5B | 202,7 | 4,05/2,13 |
LV8A | 40,1 | 0,802/0,44 |
These DNA fragments were digested with PstI. The Constructs 3B,3D 5B and 8A showed the desired bands. Hence, we transformed them in NEB-Turbo cells, by co-transformation with a PPTase. Some of the colonies (especially the colonies of LV8A) turned blue on the plates. Liquid cultures were prepared from all colonies we picked and induced with IPTG.