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Team:HokkaidoU Japan/Notebook/Promoter R0040,R0010-I732020
From 2013.igem.org
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| - | <h2></h2> | + | <h2>R0040,R0010-I732020</h2> |
<div class="workflow-container"> | <div class="workflow-container"> | ||
<ul class="steps"> | <ul class="steps"> | ||
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<div class="step-tile"><h2>Start</h2></div> | <div class="step-tile"><h2>Start</h2></div> | ||
</li> | </li> | ||
| - | + | <li class="step"> <div class="step-tile"> <h2 class="step-title">Transformation</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Fri Jul 12</time> </li> </ul> <div class="step-recipe"> R0010, R0040 from distribution kit<br> each of 2µL DNA to JM109 </div> </div> </div> </li> | |
| + | <li class="step"> <div class="step-tile"> <h2 class="step-title">Culture</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Fri Jul 12</time> </li> </ul> <div class="step-recipe"> R0010, R0040 </div> </div> </div> </li> | ||
| + | <li class="step"> <div class="step-tile"> <h2 class="step-title">Mini-prep</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Sat Jul 13</time> </li> </ul> <div class="step-recipe"> R0010, R0040 </div> </div> </div> </li> | ||
| + | <li class="step"> <div class="step-tile"> <h2 class="step-title">Digestion</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Thu Aug 1</time> </li> </ul> <div class="step-recipe"> Cut R0010, R0040 with SpeI, PstI (using 10×H buffer)<br> Cut I732020 with XbaI, PstI (using 10×M buffer) </div> </div> </div> </li> | ||
| + | <li class="step"> <div class="step-tile"> <h2 class="step-title">Antarctic phosphatase</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Thu Aug 1</time> </li> </ul> <div class="step-recipe"> R0010, R0040 </div> </div> </div> </li> | ||
| + | <li class="step"> <div class="step-tile"> <h2 class="step-title">Ligation</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Fri Aug 2</time> </li> </ul> <div class="step-recipe"> Ligate (R0010, R0040) with I732020 </div> </div> </div> </li> | ||
| + | <li class="step"> <div class="step-tile"> <h2 class="step-title">Transformation</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Fri Aug 2</time> </li> </ul> <div class="step-recipe"> (R0010, R0040)-I732020<br> each of 2µL DNA to JM109 </div> </div> </div> </li> | ||
| + | <li class="step"> <div class="step-tile"> <h2 class="step-title">Colony PCR & Culture</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Sat Aug 3</time> </li> </ul> <div class="step-recipe"> (R0010, R0040)-I732020 </div> </div> </div> </li> | ||
| + | <li class="step"> <div class="step-tile"> <h2 class="step-title">Mini-prep</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Sun Aug 4</time> </li> </ul> <div class="step-recipe"> (R0010, R0040)-I732020 </div> </div> </div> </li> | ||
<li class="step complete"> | <li class="step complete"> | ||
<div class="step-tile"><h2>Complete!</h2></div> | <div class="step-tile"><h2>Complete!</h2></div> | ||
Revision as of 07:00, 27 September 2013
Maestro E.coli
Notebook
R0040,R0010-I732020
-
Start
-
Transformation
R0010, R0040 from distribution kit
each of 2µL DNA to JM109 -
Culture
R0010, R0040 -
Mini-prep
R0010, R0040 -
Digestion
Cut R0010, R0040 with SpeI, PstI (using 10×H buffer)
Cut I732020 with XbaI, PstI (using 10×M buffer) -
Antarctic phosphatase
R0010, R0040 -
Ligation
Ligate (R0010, R0040) with I732020 -
Transformation
(R0010, R0040)-I732020
each of 2µL DNA to JM109 -
Colony PCR & Culture
(R0010, R0040)-I732020 -
Mini-prep
(R0010, R0040)-I732020 -
Complete!
