"
Team:HokkaidoU Japan/Notebook/RBS SD2,4,6-1K3-Replacement
From 2013.igem.org
(Difference between revisions)
(Created page with "{{Team:HokkaidoU_Japan/header_Maestro}} <html> <div id="common-header-bottom-background"> <div class="wrapper"> <h1 id="common-header-title">Maestro E.coli</h1> ...") |
|||
| Line 12: | Line 12: | ||
<div id="hokkaidou-contents"> | <div id="hokkaidou-contents"> | ||
<!-- end header / begin contents --> | <!-- end header / begin contents --> | ||
| - | <h2></h2> | + | <h2>SD2, 4, 6-1K3 Replacement</h2> |
<div class="workflow-container"> | <div class="workflow-container"> | ||
<ul class="steps"> | <ul class="steps"> | ||
| Line 18: | Line 18: | ||
<div class="step-tile"><h2>Start</h2></div> | <div class="step-tile"><h2>Start</h2></div> | ||
</li> | </li> | ||
| - | + | <li class="step"> <div class="step-tile"> <h2 class="step-title">PCR</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Thu Aug 15</time> </li> </ul> <div class="step-recipe"> R-0040-SD(2,4,6,8)-LacZα-B0015 100up 200dn </div> </div> </div> </li> | |
| + | <li class="step"> <div class="step-tile"> <h2 class="step-title">Digestion</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Thu Aug 15</time> </li> </ul> <div class="step-recipe"> R-0040-SD(2,4,6,8)-LacZα-B0015 EcoRI and PstI (uning 10×H buffer)<br> pSB3K3 vector EcoRI and PstI (using 10×H buffer) </div> </div> </div> </li> | ||
| + | <li class="step"> <div class="step-tile"> <h2 class="step-title">Antarctic phophatase</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Fri Aug 16</time> </li> </ul> <div class="step-recipe"> pSB3K3 vector </div> </div> </div> </li> | ||
| + | <li class="step"> <div class="step-tile"> <h2 class="step-title">Ligation</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Fri Aug 16</time> </li> </ul> <div class="step-recipe"> R-0040-SD(2,4,6,8)-LacZα-B0015 with pSB3K3 </div> </div> </div> </li> | ||
| + | <li class="step"> <div class="step-tile"> <h2 class="step-title">Transformation</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Sat Aug 17</time> </li> </ul> <div class="step-recipe"> R0040-SD(6/4/2)-LacZα-B0015<br> each of 1µL DNA to DH5α </div> </div> </div> </li> | ||
| + | <li class="step"> <div class="step-tile"> <h2 class="step-title">Mini-prep</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Tue Aug 27</time> </li> </ul> <div class="step-recipe"> R-0040-SD(2,4,6,8)-LacZα-B0015 on pSB3K3 </div> </div> </div> </li> | ||
| + | <li class="step"> <div class="step-tile"> <h2 class="step-title">Sequence</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Fri Sep 6</time> </li> </ul> <div class="step-recipe"> R-0040-SD(2,4,6,8)-LacZα-B0015 on pSB3K3 </div> </div> </div> </li> | ||
| + | <li class="step"> <div class="step-tile"> <h2 class="step-title">β-galactosidase assay (1)</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Fri Sep 20</time> </li> </ul> <div class="step-recipe"> R0040-RBS(SD2/4/6/8)-LacZα-B0015, X/S (as N. C.) </div> </div> </div> </li> | ||
| + | <li class="step"> <div class="step-tile"> <h2 class="step-title">β-galactosidase assay (2)</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Sun Sep 22</time> </li> </ul> <div class="step-recipe"> R0040-RBS(B0034/SD8)-LacZα-B0015, X/S (as N. C.) </div> </div> </div> </li> | ||
| + | <li class="step"> <div class="step-tile"> <h2 class="step-title">β-galactosidase assay (3)</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Tue Sep 24</time> </li> </ul> <div class="step-recipe"> R0040-RBS(B0034/SD8)-LacZα-B0015, X/S (as N. C.) </div> </div> </div> </li> | ||
<li class="step complete"> | <li class="step complete"> | ||
Revision as of 16:39, 27 September 2013
Maestro E.coli
Notebook
SD2, 4, 6-1K3 Replacement
-
Start
-
PCR
R-0040-SD(2,4,6,8)-LacZα-B0015 100up 200dn -
Digestion
R-0040-SD(2,4,6,8)-LacZα-B0015 EcoRI and PstI (uning 10×H buffer)
pSB3K3 vector EcoRI and PstI (using 10×H buffer) -
Antarctic phophatase
pSB3K3 vector -
Ligation
R-0040-SD(2,4,6,8)-LacZα-B0015 with pSB3K3 -
Transformation
R0040-SD(6/4/2)-LacZα-B0015
each of 1µL DNA to DH5α -
Mini-prep
R-0040-SD(2,4,6,8)-LacZα-B0015 on pSB3K3 -
Sequence
R-0040-SD(2,4,6,8)-LacZα-B0015 on pSB3K3 -
β-galactosidase assay (1)
R0040-RBS(SD2/4/6/8)-LacZα-B0015, X/S (as N. C.) -
β-galactosidase assay (2)
R0040-RBS(B0034/SD8)-LacZα-B0015, X/S (as N. C.) -
β-galactosidase assay (3)
R0040-RBS(B0034/SD8)-LacZα-B0015, X/S (as N. C.) -
Complete!
