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Team:MIT/Project
From 2013.igem.org
The MIT iGEM team sought to create a new mode of engineered intercellular communication for use in synthetic biology by modifying the contents of existing exosomes through the use of the protein tag Acyl-TyA. We built on existing research targeting proteins to exosomes to enable intercellular communication by targeting signal proteins into exosomes and into HEK 293 receiver cells.
Over the past few months we have:
Demonstrated Acyl-TyA targeting proteins to the cell membrane and into exosomes
Designed a number of reporter constructs to assay for our signals:
Designed Acyl-TyA fusion proteins with our signals:
Demonstrated native exosomal microRNA repression with isolated exosomes and Jukat/HEK293 coculture experiments.
Demonstrated activation of a reporter using the trans activator Cas9-VP16.
Demonstrated DNA sensing using Cas9-Split Venus reconstitution.
Over the past few months we have:
- rtTA3
- L7Ae
- Cas9-VP16
- Cas9-Split Venus
- Cre Recombinase
- rtTA3
- L7Ae
- Cas9-VP16
- Cas9-Split Venus
- Cre Recombinase
