Team:Manchester/LabBooktest
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- | <li id="one"><a href="" onclick="blocking('text'); return false;"><span id="arrow"><img src="https://static.igem.org/mediawiki/2013/e/ee/DownArrow2.png" width="30" height="30"/></span><span id="date"> | + | <li id="one"><a href="" onclick="blocking('text'); return false;"><span id="arrow"><img src="https://static.igem.org/mediawiki/2013/e/ee/DownArrow2.png" width="30" height="30"/></span><span id="date">14/06/2013 </span> Making media</a> |
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- | <li id="one"><a href="" onclick="blocking('text4'); return false;"><span id="arrow"><img src="https://static.igem.org/mediawiki/2013/e/ee/DownArrow2.png" width="30" height="30"/></span><span id="date"> | + | <li id="one"><a href="" onclick="blocking('text4'); return false;"><span id="arrow"><img src="https://static.igem.org/mediawiki/2013/e/ee/DownArrow2.png" width="30" height="30"/></span><span id="date">21/06/2013</span> Continued</a> |
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- | <li id="one"><a href="" onclick="blocking('text5'); return false;"><span id="arrow"><img src="https://static.igem.org/mediawiki/2013/e/ee/DownArrow2.png" width="30" height="30"/></span><span id="date"> | + | <li id="one"><a href="" onclick="blocking('text5'); return false;"><span id="arrow"><img src="https://static.igem.org/mediawiki/2013/e/ee/DownArrow2.png" width="30" height="30"/></span><span id="date">24/06/2013</span> Growing Cells From 21/06/2013 </a> |
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- | <li id="one"><a href="" onclick="blocking('text6'); return false;"><span id="arrow"><img src="https://static.igem.org/mediawiki/2013/e/ee/DownArrow2.png" width="30" height="30"/></span><span id="date"> | + | <li id="one"><a href="" onclick="blocking('text6'); return false;"><span id="arrow"><img src="https://static.igem.org/mediawiki/2013/e/ee/DownArrow2.png" width="30" height="30"/></span><span id="date">25/06/2013</span> Electrically Competent Cells For Electroporation (E.Coli BL21 DE3) </a> |
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- | <li id="one"><a href="" onclick="blocking('text7'); return false;"><span id="arrow"><img src="https://static.igem.org/mediawiki/2013/e/ee/DownArrow2.png" width="30" height="30"/></span><span id="date"> | + | <li id="one"><a href="" onclick="blocking('text7'); return false;"><span id="arrow"><img src="https://static.igem.org/mediawiki/2013/e/ee/DownArrow2.png" width="30" height="30"/></span><span id="date">26/06/2013 </span> Electroporation of PKD46 in E.Coli BL21 DE3 from 25/06/2013</a> |
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- | <li id="one"><a href="" onclick="blocking('text12'); return false;"><span id="arrow"><img src="https://static.igem.org/mediawiki/2013/e/ee/DownArrow2.png" width="30" height="30"/></span><span id="date"> | + | <li id="one"><a href="" onclick="blocking('text12'); return false;"><span id="arrow"><img src="https://static.igem.org/mediawiki/2013/e/ee/DownArrow2.png" width="30" height="30"/></span><span id="date">04/07/2013</span> Induction of Lambda Red Recombinase & Electro-Competency of transformed cells</a> |
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- | <li id="one"><a href="" onclick="blocking('text13'); return false;"><span id="arrow"><img src="https://static.igem.org/mediawiki/2013/e/ee/DownArrow2.png" width="30" height="30"/></span><span id="date"> | + | <li id="one"><a href="" onclick="blocking('text13'); return false;"><span id="arrow"><img src="https://static.igem.org/mediawiki/2013/e/ee/DownArrow2.png" width="30" height="30"/></span><span id="date">09/07/2013</span> PCR of Chloramphenicol and Homologous regions </a> |
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- | <li id="one"><a href="" onclick="blocking('text14'); return false;"><span id="arrow"><img src="https://static.igem.org/mediawiki/2013/e/ee/DownArrow2.png" width="30" height="30"/></span><span id="date"> | + | <li id="one"><a href="" onclick="blocking('text14'); return false;"><span id="arrow"><img src="https://static.igem.org/mediawiki/2013/e/ee/DownArrow2.png" width="30" height="30"/></span><span id="date">10/07/2013</span> Agarose Gel Electrophoresis of PCR product from 09/07</a> |
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+ | |||
+ | <li id="one"><a href="" onclick="blocking('text15'); return false;"><span id="arrow"><img src="https://static.igem.org/mediawiki/2013/e/ee/DownArrow2.png" width="30" height="30"/></span><span id="date">11/07/2013 </span> Received FAS Module - Plating up</a> | ||
+ | <div id="text15"> | ||
+ | <p> | ||
+ | <p>2 vials of stab culture received from Prof Mattheos Koffas of the Rensselaer Polytechnic Institute, NY, USA (2x DH5α w/pETM6-CnFatB2-fabAH<sup>*</sup>GI in Amp80</p> | ||
+ | <p>1 vial kept at 4 ºC and other vial plated on LB with Amp100</p> | ||
+ | <p>Left on bench for 3 hours and then placed in a 30 ºC incubator</p> | ||
+ | <p>Second plate, Amp 50µg/ml, plated w/DH5α w/pETM6-CnFatB2-fabAH<sup>*</sup>GI and placed in 30 ºC incubator</p> | ||
+ | <p>Both plates removed and left on bench overnight</p> | ||
+ | </p> | ||
+ | </div> | ||
+ | </li> | ||
+ | |||
+ | <li id="one"><a href="" onclick="blocking('text16'); return false;"><span id="arrow"><img src="https://static.igem.org/mediawiki/2013/e/ee/DownArrow2.png" width="30" height="30"/></span><span id="date">15/07/2013 </span> Stock of FAS cells for freezer</a> | ||
+ | <div id="text16"> | ||
+ | <p> | ||
+ | <p>Successful growth found on the plates over the weekend. Stock made using the following protocol:</p> | ||
+ | <p>1) Make 10 ml of LB and 100 µg/ml of Ampicillin</p> | ||
+ | <p>2) Inoculate media with cells on plates w/FAS</p> | ||
+ | <p>3) Grow at 37 ºC for 5 hours</p> | ||
+ | <p>4) Spin cells down in centrifuge</p> | ||
+ | <p>5) Resuspend in 700 µl of LB and glycerol</p> | ||
+ | <br> | ||
+ | <p><b>18/07/2013: Growing up DH5α for plasmid extraction</b></p> | ||
+ | <p>1) 100 ml of LB w/100 µg/ml Ampicillin</p> | ||
+ | <p>2) Inoculate with cells</p> | ||
+ | <p>3) Leave to grow at 37 ºC overnight</p> | ||
+ | <br> | ||
+ | <p><b>18/07/2013: Extraction of FAS module</b></p> | ||
+ | <p>FAS module successfully extracted using plasmid purification kit</p> | ||
+ | <p><b>INSERT PICTURE OF GEL HERE</b></p> | ||
+ | <p><b>19/07/2013-24th July: New Primers</b></p> | ||
+ | <p>Found that the PCR from 9/7/2013 failed due to incorrect primers (H1catF and H2catR). New primers were ordered:</p> | ||
+ | <p><i>H1catF_2a</i></p> | ||
+ | <p>CGGCATGTATATCATTTGGGGTTGCGATGACGACGAACACGCATT</p> | ||
+ | <p><i>H1catR_2a</i></p> | ||
+ | <p>CTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTG</p> | ||
+ | </div> | ||
+ | </li> | ||
+ | |||
</ul> | </ul> | ||
Revision as of 11:00, 2 October 2013