Team:NCTU Formosa/biobricks

Light-regulated system

BBa_K1017726

BBa_K1017301

BBa_K1017781

BBa_K1017101

• Ompc promtor, which can sense red light with the presence of cph8. It will be turned on in the dark ,and be turned off in the bright. For the convenient use, we add lacI and lac promotor downstream the biobrick. This part, so called red promotor, can be activated under red light, and inactive in the dark.

Temperature-regulated system

BBa_K1017602

• By using mGFP as a reporter gene, we can test whether the 37 °C RBS works.

BBa_K1017603

• In our circuit, this biobrick is the part of P_lux's activation when the temperature reaches to 37^oC.

Small RNA-regulated system

BBa_K1017403

BBa_K1017404

BBa_K1017202

• The sRNA, base pair with target mRNA, including the Shine-Dalgarno sequence. Thus it prevent ribosome from binding to initiate the translation. The rRBS is designed for sRNA perfect binding, and this rRBS is the RBS which can be bound only for our artificial sRNA(BBa_K1017404).

BBa_K1017401

• This part, BBa_K1017401, includes our artificial sRNA-1 and rRBS-1. The non-coding small RNA can bind to the Shine-Dalgarno sequence on rRBS-1 by base-pairing. Once the rRBS-1 is blocked, ribosomes cannot bind to it to translate, thus, gene expressions downstream are decreased. Because of specific binding, rRBS-1 can only be bound by sRNA-1. We add P_lux upstream, so this part can be regulated by luxR/AHL complex.

BBa_K1017402

• This part, BBa_K1017402, is similar to BBa_K1017401 mentioned above, but without P_lux. rRBS-2 can only be bound by sRNA-2 due to specificity, then gene expressions downstream are decreased.

BBa_K1017811