Team:NTNU-Trondheim/Primers

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(Difference between revisions)

Latest revision as of 20:59, 4 October 2013

Trondheim iGEM 2013

Primers

tat-signal sequence
Primer	Sequence
F_pl.b_tat
5'- AGAGAAAGAGGAGAAATACTAGatggccaataacgatctctttcaggcatcacg-3'
F_prefix_tat
5'- GAATTCGCGGCCGCTTCTAGatggccaataacgatctctttcaggcatcacg-3'
R_tat
5'-cgcttgcgccgcagtcgcacgtcg-3'
R_GFP_tat
5'-CTTCTCCTTTACGCATcgcttgcgccgcagtcgcacgtcg -3'
R_CFP_tat
5'-GCCCTTGCTCACCATcgcttgcgccgcagtcgcacgtcg-3'
SYFP
F_I_syfp
5'-GGTGGATCCGGTGGATCAGGTGGATCCGGTGGTAGTatggttagcaagggcgaaga-3'
R_syfp
5'-GCCTTTCGTTTTATTTGATGCCTGGttattatttatacagctcatccatacccaggg-3'
BFP
F_tat_BFP
5'- CGACGTGCGACTGCGGCGCAAGCGatgagcgaactgatcaaagag-3'
R_I_bfp
5'-ACTACCACCGGATCCACCTGATCCACCGGATCCACCattcagtttatgacccagcttgc-3'
GFP
F_tat_GFP
5'-CGACGTGCGACTGCGGCGCAAGCGatgcgtaaaggagaagaact-3'
R_I_gfp
5'-ACTACCACCGGATCCACCTGATCCACCGGATCCACCtttgtatagttcatccatgcc-3'
RFP
F_I_rfp
5'- GGTGGATCCGGTGGATCAGGTGGATCCGGTGGTAGTatggcttcctccgaagacg-3'
R_pl.b_rfp
5'- GCCTTTCGTTTTATTTGATGCCTGGgcgatctacactagcactatcagcg-3'
GFPmut3
F_tat_GFPmut3
5'-CGACGTGCGACTGCGGCGCAAGCGagaaaaggagaagaacttttcactgg-3'
R_pl.b_GFPmut3
5-'GCCTTTCGTTTTATTTGATGCCTGGttatttgtatagttcatccatgcc-3'
CFP
F_tat_CFP
5'-CGACGTGCGACTGCGGCGCAAGCGatggtgagcaagggcgaggagctgttc-3'
R_l_CFP
5'- ACTACCACCGGATCCACCTGATCCACCGGATCCACCcttgtacagctcgtccatgccgagag-3'
YFP
F_l_YFP
5'-GGTGGATCCGGTGGATCAGGTGGATCCGGTGGTAGTatggtgagcaagggcgaggagctgttc-3'
R_pl.b_YFP
5'- GCCTTTCGTTTTATTTGATGCCTGGttattacttgtacagctcgtccatgccg-3'
Protein G
F_pl.b_PrG
5'-AGAGAAAGAGGAGAAATACTAGatggaaaaagaaaaaaaggtaaaatac-3'
F_tat_PrG
5'-CGACGTGCGACTGCGGCGCAAGCGgttgactcaccaatcgaagatacccc-3'
F_pl.b_pelB_PrB
5'-AGAGAAAGAGGAGAAATACTAGATGAAATACCTGCTGCCGACCGCTGCTGCTGGTCTGCTGCTCCTCGCTGCCCAGCCGGCGATGGCC gttgactcaccaatcgaagatacccc-3'
R_pl.b_PrG
5'- GCCTTTCGTTTTATTTGATGCCTGGgatcggcatattgaaaaggc-3'
R_pl.b_PrGstop
5'-GCCTTTCGTTTTATTTGATGCCTGGttagtcttctttacgttttgaagcgac-3'
Plasmid backbone
F_plasmid
5'-ccaggcatcaaataaaacgaaaggc-3'
F_RFP_pl.b
5'-GTGCTAGTGTAGATCGCccaggcatcaaataaaacgaaaggc-3'
F_YFP_pl.b
5'-GAGCTGTACAAGTAATAAccaggcatcaaataaaacgaaaggc-3'
R_pl.b
5'- ctagtatttctcctctttctctagtagtgc-3'
R_tat_pl.b
5'- GAGATCGTTATTGTTCATctagtatttctcctctttctctagtagtgc-3'
Sequencing Primers
F_sequence
5'-tgccacctgacgtctaagaa-3'
R_sequence
5'-attaccgcctttgagtgagc-3'

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-<div id="top-section3"><center><img src="https://static.igem.org/mediawiki/2013/5/55/Logo-ntnu.png" alt="header" border="0"  usemap="#igemmap" width="1000" height="400"></center></div>
+<div id="top-section3"><center><img src="https://static.igem.org/mediawiki/2013/2/21/Logo2_NTNU.png" alt="header" border="0"  usemap="#igemmap" width="1000" height="400"></center></div>
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 <p style="text-align:center; color:#1A1719; "> Primers </p> </div>
 <br><br>
 <p>
+<table style="border:1px solid black;">
+<tr>
+<th> tat-signal sequence</th>
+</tr>
+<tr>
+<th>Primer</th>
+<th>Sequence</th>
+</tr>
+<tr>
+<td><b> F_pl.b_tat</tr></b></td>
+<td>5'- AGAGAAAGAGGAGAAATACTAGatggccaataacgatctctttcaggcatcacg-3'</td>
+</tr>
+<tr>
+<td><b> F_prefix_tat</tr></b></td>
+<td>5'- GAATTCGCGGCCGCTTCTAGatggccaataacgatctctttcaggcatcacg-3'</td>
+</tr>
+<tr>
+<td><b> R_tat</tr></b></td>
+<td>5'-cgcttgcgccgcagtcgcacgtcg-3'</td>
+</tr>
+<tr>
+<td><b> R_GFP_tat</tr></b></td>
+<td>5'-CTTCTCCTTTACGCATcgcttgcgccgcagtcgcacgtcg -3'</td>
+</tr>
+<tr>
+<td><b> R_CFP_tat</tr></b></td>
+<td>5'-GCCCTTGCTCACCATcgcttgcgccgcagtcgcacgtcg-3'</td>
+</tr>
+<tr>
+<th> SYFP</th>
+</tr>
+<tr>
+<td><b> F_I_syfp</tr></b></td>
+<td>5'-GGTGGATCCGGTGGATCAGGTGGATCCGGTGGTAGTatggttagcaagggcgaaga-3'</td>
+</tr>
+<tr>
+<td><b> R_syfp</tr></b></td>
+<td>5'-GCCTTTCGTTTTATTTGATGCCTGGttattatttatacagctcatccatacccaggg-3'</td>
+</tr>
+<tr>
+<th> BFP</th>
+<tr>
+<td><b> F_tat_BFP</tr></b></td>
+<td>5'- CGACGTGCGACTGCGGCGCAAGCGatgagcgaactgatcaaagag-3' </td>
+</tr>
+<tr>
+<td><b>R_I_bfp</tr></b></td>
+<td>5'-ACTACCACCGGATCCACCTGATCCACCGGATCCACCattcagtttatgacccagcttgc-3'</td>
+</tr>
+<tr>
+<th> GFP</th>
+<tr>
+<td><b>F_tat_GFP</tr></b></td>
+<td>5'-CGACGTGCGACTGCGGCGCAAGCGatgcgtaaaggagaagaact-3' </td>
+</tr>
+<tr>
+<td><b>R_I_gfp</tr></b></td>
+<td>5'-ACTACCACCGGATCCACCTGATCCACCGGATCCACCtttgtatagttcatccatgcc-3'</td>
+</tr>
+<tr>
+<th> RFP</th>
+<tr>
+<td><b>F_I_rfp</tr></b></td>
+<td>5'- GGTGGATCCGGTGGATCAGGTGGATCCGGTGGTAGTatggcttcctccgaagacg-3'</td>
+</tr>
+<tr>
+<td><b>R_pl.b_rfp</tr></b></td>
+<td>5'- GCCTTTCGTTTTATTTGATGCCTGGgcgatctacactagcactatcagcg-3'</td>
+</tr>
+<tr>
+<th> GFPmut3</th>
+<tr>
+<td><b>F_tat_GFPmut3</tr></b></td>
+<td>5'-CGACGTGCGACTGCGGCGCAAGCGagaaaaggagaagaacttttcactgg-3'</td>
+</tr>
+<tr>
+<td><b>R_pl.b_GFPmut3</tr></b></td>
+<td>5-'GCCTTTCGTTTTATTTGATGCCTGGttatttgtatagttcatccatgcc-3'</td>
+</tr>
+<tr>
+<th> CFP</th>
+<tr>
+<td><b>F_tat_CFP</tr></b></td>
+<td>5'-CGACGTGCGACTGCGGCGCAAGCGatggtgagcaagggcgaggagctgttc-3'</td>
+</tr>
+<tr>
+<td><b>R_l_CFP</tr></b></td>
+<td>5'- ACTACCACCGGATCCACCTGATCCACCGGATCCACCcttgtacagctcgtccatgccgagag-3'</td>
+</tr>
+<tr>
+<th> YFP</th>
+<tr>
+<td><b>F_l_YFP</tr></b></td>
+<td>5'-GGTGGATCCGGTGGATCAGGTGGATCCGGTGGTAGTatggtgagcaagggcgaggagctgttc-3'</td>
+</tr>
+<tr>
+<td><b>R_pl.b_YFP</tr></b></td>
+<td>5'- GCCTTTCGTTTTATTTGATGCCTGGttattacttgtacagctcgtccatgccg-3'</td>
+</tr>
+<tr>
+<th> Protein G</th>
+<tr>
+<td><b>F_pl.b_PrG</tr></b></td>
+<td>5'-AGAGAAAGAGGAGAAATACTAGatggaaaaagaaaaaaaggtaaaatac-3'</td>
+</tr>
+<tr>
+<td><b>F_tat_PrG</tr></b></td>
+<td>5'-CGACGTGCGACTGCGGCGCAAGCGgttgactcaccaatcgaagatacccc-3'</td>
+</tr>
+<tr>
+<td><b>F_pl.b_pelB_PrB</tr></b></td>
+<td><p>5'-AGAGAAAGAGGAGAAATACTAGATGAAATACCTGCTGCCGACCGCTGCTGCTGGTCTGCTGCTCCTCGCTGCCCAGCCGGCGATGGCC</p>
+<p>gttgactcaccaatcgaagatacccc-3'</p></td>
+</tr>
+<tr>
+<td><b>R_pl.b_PrG</tr></b></td>
+<td>5'- GCCTTTCGTTTTATTTGATGCCTGGgatcggcatattgaaaaggc-3'</td>
+</tr>
+<tr>
+<td><b>R_pl.b_PrGstop</tr></b></td>
+<td>5'-GCCTTTCGTTTTATTTGATGCCTGGttagtcttctttacgttttgaagcgac-3'</td>
+</tr>
+<tr>
+<th> Plasmid backbone</th>
+<tr>
+<td><b>F_plasmid </tr></b></td>
+<td>5'-ccaggcatcaaataaaacgaaaggc-3'</td>
+</tr>
+<tr>
+<td><b>F_RFP_pl.b</tr></b></td>
+<td>5'-GTGCTAGTGTAGATCGCccaggcatcaaataaaacgaaaggc-3'</td>
+</tr>
+<tr>
+<td><b>F_YFP_pl.b</tr></b></td>
+<td>5'-GAGCTGTACAAGTAATAAccaggcatcaaataaaacgaaaggc-3'</td>
+</tr>
+<tr>
+<td><b>R_pl.b</tr></b></td>
+<td>5'- ctagtatttctcctctttctctagtagtgc-3'</td>
+</tr>
+<tr>
+<td><b>R_tat_pl.b</tr></b></td>
+<td>5'- GAGATCGTTATTGTTCATctagtatttctcctctttctctagtagtgc-3'</td>
+</tr>
+<tr>
+<th> Sequencing Primers</th>
+<tr>
+<td><b>F_sequence</tr></b></td>
+<td>5'-tgccacctgacgtctaagaa-3'</td>
+</tr>
+<tr>
+<td><b>R_sequence</tr></b></td>
+<td>5'-attaccgcctttgagtgagc-3'</td>
+</tr>
+</table>