Team:NTNU-Trondheim/Primers

From 2013.igem.org

(Difference between revisions)
 
(10 intermediate revisions not shown)
Line 19: Line 19:
-
<div id="top-section3"><center><img src="https://static.igem.org/mediawiki/2013/5/55/Logo-ntnu.png" alt="header" border="0"  usemap="#igemmap" width="1000" height="400"></center></div>
+
<div id="top-section3"><center><img src="https://static.igem.org/mediawiki/2013/2/21/Logo2_NTNU.png" alt="header" border="0"  usemap="#igemmap" width="1000" height="400"></center></div>
Line 33: Line 33:
<div id='cssmenu'>
<div id='cssmenu'>
<ul>
<ul>
-
   <li><a href='https://2013.igem.org/Team:NTNU-Trondheim'><span>Home</span></a></li>
+
   <li class='active'><a href='https://2013.igem.org/Team:NTNU-Trondheim'><span>Home</span></a></li>
   <li class='has-sub'><a href='#'><span>Project</span></a>
   <li class='has-sub'><a href='#'><span>Project</span></a>
       <ul>
       <ul>
         <li><a href='https://2013.igem.org/Team:NTNU-Trondheim/Project'><span>Project description</span></a></li>
         <li><a href='https://2013.igem.org/Team:NTNU-Trondheim/Project'><span>Project description</span></a></li>
 +
        <li><a href='https://2013.igem.org/Team:NTNU-Trondheim/novelapproach'><span>A novel approach</span></a></li>
         <li><a href='https://2013.igem.org/Team:NTNU-Trondheim/Model'><span>Modelling</span></a></li>
         <li><a href='https://2013.igem.org/Team:NTNU-Trondheim/Model'><span>Modelling</span></a></li>
         <li><a href='https://2013.igem.org/Team:NTNU-Trondheim/Experiments_and_Results'><span>Experiments and Results</span></a></li>
         <li><a href='https://2013.igem.org/Team:NTNU-Trondheim/Experiments_and_Results'><span>Experiments and Results</span></a></li>
         <li><a href='https://2013.igem.org/Team:NTNU-Trondheim/Parts'><span>BioBrick Parts</span></a></li>
         <li><a href='https://2013.igem.org/Team:NTNU-Trondheim/Parts'><span>BioBrick Parts</span></a></li>
-
         <li class='last'><a href='https://2013.igem.org/Team:NTNU_Trondheim/Attributions'><span>Attributions</span></a></li>
+
         <li class='last'><a href='https://2013.igem.org/Team:NTNU-Trondheim/Acknowledgements'><span>Acknowledgements</span></a></li>
       </ul>
       </ul>
   </li>
   </li>
Line 59: Line 60:
   <li class='has-sub'><a href='https://2013.igem.org/Team:NTNU-Trondheim/Outreach'><span>Outreach</span></a>
   <li class='has-sub'><a href='https://2013.igem.org/Team:NTNU-Trondheim/Outreach'><span>Outreach</span></a>
   </li>
   </li>
-
  <li><a href='https://2013.igem.org/Team:NTNU-Trondheim/Achievements'><span>Achievements</span></a></li>
+
    <li class='has-sub'><a href='#'><span>Judging</span></a>
 +
    <ul>
 +
    <li><a href='https://2013.igem.org/Team:NTNU-Trondheim/Achievements'><span>Achievements</span></a></li>
 +
    <li class='last'><a href='https://2013.igem.org/Team:NTNU-Trondheim/Medalcriteria'><span>Medal criteria</span></a></li>
 +
    </ul>
 +
    </li>
   <li class='last'><a href='https://2012.igem.org/Team:NTNU_Trondheim/Matchmaker'><span>Matchmaker</span></a></li>
   <li class='last'><a href='https://2012.igem.org/Team:NTNU_Trondheim/Matchmaker'><span>Matchmaker</span></a></li>
</ul>
</ul>
-
  </div>
+
</div>
<div> <div class ="row-end"> </div>
<div> <div class ="row-end"> </div>
</div>
</div>
Line 69: Line 75:
<div class="row">
<div class="row">
<div class="col12-2">  
<div class="col12-2">  
-
<div class="col8" align = "justify" style="background-color:#7352B0;>
+
<div class="col8" align = "justify" style="background-color:#C3A7F3;>
<p style="text-align:center; color:#1A1719; "> Primers </p> </div>
<p style="text-align:center; color:#1A1719; "> Primers </p> </div>
<br><br>
<br><br>
Line 80: Line 86:
<tr>
<tr>
<th>Primer</th>
<th>Primer</th>
-
<th>Sequance</th>
+
<th>Sequence</th>
</tr>
</tr>
<tr>
<tr>
Line 108: Line 114:
<tr>
<tr>
<td><b> F_I_syfp</tr></b></td>
<td><b> F_I_syfp</tr></b></td>
-
<td></td>
+
<td>5'-GGTGGATCCGGTGGATCAGGTGGATCCGGTGGTAGTatggttagcaagggcgaaga-3'</td>
</tr>
</tr>
<tr>
<tr>
<td><b> R_syfp</tr></b></td>
<td><b> R_syfp</tr></b></td>
-
<td></td>
+
<td>5'-GCCTTTCGTTTTATTTGATGCCTGGttattatttatacagctcatccatacccaggg-3'</td>
</tr>
</tr>
 +
<tr>
<tr>
<th> BFP</th>
<th> BFP</th>
<tr>
<tr>
<td><b> F_tat_BFP</tr></b></td>
<td><b> F_tat_BFP</tr></b></td>
-
<td></td>
+
<td>5'- CGACGTGCGACTGCGGCGCAAGCGatgagcgaactgatcaaagag-3' </td>
</tr>
</tr>
<tr>
<tr>
<td><b>R_I_bfp</tr></b></td>
<td><b>R_I_bfp</tr></b></td>
-
<td></td>
+
<td>5'-ACTACCACCGGATCCACCTGATCCACCGGATCCACCattcagtttatgacccagcttgc-3'</td>
</tr>
</tr>
Line 129: Line 136:
<tr>
<tr>
<td><b>F_tat_GFP</tr></b></td>
<td><b>F_tat_GFP</tr></b></td>
-
<td></td>
+
<td>5'-CGACGTGCGACTGCGGCGCAAGCGatgcgtaaaggagaagaact-3' </td>
</tr>
</tr>
<tr>
<tr>
<td><b>R_I_gfp</tr></b></td>
<td><b>R_I_gfp</tr></b></td>
-
<td></td>
+
<td>5'-ACTACCACCGGATCCACCTGATCCACCGGATCCACCtttgtatagttcatccatgcc-3'</td>
</tr>
</tr>
Line 140: Line 147:
<tr>
<tr>
<td><b>F_I_rfp</tr></b></td>
<td><b>F_I_rfp</tr></b></td>
-
<td></td>
+
<td>5'- GGTGGATCCGGTGGATCAGGTGGATCCGGTGGTAGTatggcttcctccgaagacg-3'</td>
</tr>
</tr>
<tr>
<tr>
<td><b>R_pl.b_rfp</tr></b></td>
<td><b>R_pl.b_rfp</tr></b></td>
-
<td></td>
+
<td>5'- GCCTTTCGTTTTATTTGATGCCTGGgcgatctacactagcactatcagcg-3'</td>
</tr>
</tr>
Line 151: Line 158:
<tr>
<tr>
<td><b>F_tat_GFPmut3</tr></b></td>
<td><b>F_tat_GFPmut3</tr></b></td>
-
<td></td>
+
<td>5'-CGACGTGCGACTGCGGCGCAAGCGagaaaaggagaagaacttttcactgg-3'</td>
</tr>
</tr>
<tr>
<tr>
<td><b>R_pl.b_GFPmut3</tr></b></td>
<td><b>R_pl.b_GFPmut3</tr></b></td>
-
<td></td>
+
<td>5-'GCCTTTCGTTTTATTTGATGCCTGGttatttgtatagttcatccatgcc-3'</td>
</tr>
</tr>
Line 162: Line 169:
<tr>
<tr>
<td><b>F_tat_CFP</tr></b></td>
<td><b>F_tat_CFP</tr></b></td>
-
<td></td>
+
<td>5'-CGACGTGCGACTGCGGCGCAAGCGatggtgagcaagggcgaggagctgttc-3'</td>
</tr>
</tr>
<tr>
<tr>
<td><b>R_l_CFP</tr></b></td>
<td><b>R_l_CFP</tr></b></td>
-
<td></td>
+
<td>5'- ACTACCACCGGATCCACCTGATCCACCGGATCCACCcttgtacagctcgtccatgccgagag-3'</td>
</tr>
</tr>
Line 173: Line 180:
<tr>
<tr>
<td><b>F_l_YFP</tr></b></td>
<td><b>F_l_YFP</tr></b></td>
-
<td></td>
+
<td>5'-GGTGGATCCGGTGGATCAGGTGGATCCGGTGGTAGTatggtgagcaagggcgaggagctgttc-3'</td>
</tr>
</tr>
<tr>
<tr>
<td><b>R_pl.b_YFP</tr></b></td>
<td><b>R_pl.b_YFP</tr></b></td>
-
<td></td>
+
<td>5'- GCCTTTCGTTTTATTTGATGCCTGGttattacttgtacagctcgtccatgccg-3'</td>
</tr>
</tr>
Line 184: Line 191:
<tr>
<tr>
<td><b>F_pl.b_PrG</tr></b></td>
<td><b>F_pl.b_PrG</tr></b></td>
-
<td></td>
+
<td>5'-AGAGAAAGAGGAGAAATACTAGatggaaaaagaaaaaaaggtaaaatac-3'</td>
</tr>
</tr>
<tr>
<tr>
<td><b>F_tat_PrG</tr></b></td>
<td><b>F_tat_PrG</tr></b></td>
-
<td></td>
+
<td>5'-CGACGTGCGACTGCGGCGCAAGCGgttgactcaccaatcgaagatacccc-3'</td>
</tr>
</tr>
<tr>
<tr>
<td><b>F_pl.b_pelB_PrB</tr></b></td>
<td><b>F_pl.b_pelB_PrB</tr></b></td>
-
<td></td>
+
<td><p>5'-AGAGAAAGAGGAGAAATACTAGATGAAATACCTGCTGCCGACCGCTGCTGCTGGTCTGCTGCTCCTCGCTGCCCAGCCGGCGATGGCC</p>
 +
<p>gttgactcaccaatcgaagatacccc-3'</p></td>
</tr>
</tr>
<tr>
<tr>
<td><b>R_pl.b_PrG</tr></b></td>
<td><b>R_pl.b_PrG</tr></b></td>
-
<td></td>
+
<td>5'- GCCTTTCGTTTTATTTGATGCCTGGgatcggcatattgaaaaggc-3'</td>
</tr>
</tr>
<tr>
<tr>
<td><b>R_pl.b_PrGstop</tr></b></td>
<td><b>R_pl.b_PrGstop</tr></b></td>
-
<td></td>
+
<td>5'-GCCTTTCGTTTTATTTGATGCCTGGttagtcttctttacgttttgaagcgac-3'</td>
</tr>
</tr>
Line 207: Line 215:
<tr>
<tr>
<td><b>F_plasmid </tr></b></td>
<td><b>F_plasmid </tr></b></td>
-
<td></td>
+
<td>5'-ccaggcatcaaataaaacgaaaggc-3'</td>
</tr>
</tr>
<tr>
<tr>
<td><b>F_RFP_pl.b</tr></b></td>
<td><b>F_RFP_pl.b</tr></b></td>
-
<td></td>
+
<td>5'-GTGCTAGTGTAGATCGCccaggcatcaaataaaacgaaaggc-3'</td>
</tr>
</tr>
<tr>
<tr>
<td><b>F_YFP_pl.b</tr></b></td>
<td><b>F_YFP_pl.b</tr></b></td>
-
<td></td>
+
<td>5'-GAGCTGTACAAGTAATAAccaggcatcaaataaaacgaaaggc-3'</td>
</tr>
</tr>
<tr>
<tr>
<td><b>R_pl.b</tr></b></td>
<td><b>R_pl.b</tr></b></td>
-
<td></td>
+
<td>5'- ctagtatttctcctctttctctagtagtgc-3'</td>
</tr>
</tr>
<tr>
<tr>
<td><b>R_tat_pl.b</tr></b></td>
<td><b>R_tat_pl.b</tr></b></td>
-
<td></td>
+
<td>5'- GAGATCGTTATTGTTCATctagtatttctcctctttctctagtagtgc-3'</td>
</tr>
</tr>
<tr>
<tr>
-
<th> Sequancing Primers</th>
+
<th> Sequencing Primers</th>
<tr>
<tr>
<td><b>F_sequence</tr></b></td>
<td><b>F_sequence</tr></b></td>
-
<td></td>
+
<td>5'-tgccacctgacgtctaagaa-3'</td>
</tr>
</tr>
<tr>
<tr>
<td><b>R_sequence</tr></b></td>
<td><b>R_sequence</tr></b></td>
-
<td></td>
+
<td>5'-attaccgcctttgagtgagc-3'</td>
</tr>
</tr>

Latest revision as of 20:59, 4 October 2013

Trondheim iGEM 2013

header
Mercury
Primers



tat-signal sequence
Primer Sequence
F_pl.b_tat
5'- AGAGAAAGAGGAGAAATACTAGatggccaataacgatctctttcaggcatcacg-3'
F_prefix_tat
5'- GAATTCGCGGCCGCTTCTAGatggccaataacgatctctttcaggcatcacg-3'
R_tat
5'-cgcttgcgccgcagtcgcacgtcg-3'
R_GFP_tat
5'-CTTCTCCTTTACGCATcgcttgcgccgcagtcgcacgtcg -3'
R_CFP_tat
5'-GCCCTTGCTCACCATcgcttgcgccgcagtcgcacgtcg-3'
SYFP
F_I_syfp
5'-GGTGGATCCGGTGGATCAGGTGGATCCGGTGGTAGTatggttagcaagggcgaaga-3'
R_syfp
5'-GCCTTTCGTTTTATTTGATGCCTGGttattatttatacagctcatccatacccaggg-3'
BFP
F_tat_BFP
5'- CGACGTGCGACTGCGGCGCAAGCGatgagcgaactgatcaaagag-3'
R_I_bfp
5'-ACTACCACCGGATCCACCTGATCCACCGGATCCACCattcagtttatgacccagcttgc-3'
GFP
F_tat_GFP
5'-CGACGTGCGACTGCGGCGCAAGCGatgcgtaaaggagaagaact-3'
R_I_gfp
5'-ACTACCACCGGATCCACCTGATCCACCGGATCCACCtttgtatagttcatccatgcc-3'
RFP
F_I_rfp
5'- GGTGGATCCGGTGGATCAGGTGGATCCGGTGGTAGTatggcttcctccgaagacg-3'
R_pl.b_rfp
5'- GCCTTTCGTTTTATTTGATGCCTGGgcgatctacactagcactatcagcg-3'
GFPmut3
F_tat_GFPmut3
5'-CGACGTGCGACTGCGGCGCAAGCGagaaaaggagaagaacttttcactgg-3'
R_pl.b_GFPmut3
5-'GCCTTTCGTTTTATTTGATGCCTGGttatttgtatagttcatccatgcc-3'
CFP
F_tat_CFP
5'-CGACGTGCGACTGCGGCGCAAGCGatggtgagcaagggcgaggagctgttc-3'
R_l_CFP
5'- ACTACCACCGGATCCACCTGATCCACCGGATCCACCcttgtacagctcgtccatgccgagag-3'
YFP
F_l_YFP
5'-GGTGGATCCGGTGGATCAGGTGGATCCGGTGGTAGTatggtgagcaagggcgaggagctgttc-3'
R_pl.b_YFP
5'- GCCTTTCGTTTTATTTGATGCCTGGttattacttgtacagctcgtccatgccg-3'
Protein G
F_pl.b_PrG
5'-AGAGAAAGAGGAGAAATACTAGatggaaaaagaaaaaaaggtaaaatac-3'
F_tat_PrG
5'-CGACGTGCGACTGCGGCGCAAGCGgttgactcaccaatcgaagatacccc-3'
F_pl.b_pelB_PrB

5'-AGAGAAAGAGGAGAAATACTAGATGAAATACCTGCTGCCGACCGCTGCTGCTGGTCTGCTGCTCCTCGCTGCCCAGCCGGCGATGGCC

gttgactcaccaatcgaagatacccc-3'

R_pl.b_PrG
5'- GCCTTTCGTTTTATTTGATGCCTGGgatcggcatattgaaaaggc-3'
R_pl.b_PrGstop
5'-GCCTTTCGTTTTATTTGATGCCTGGttagtcttctttacgttttgaagcgac-3'
Plasmid backbone
F_plasmid
5'-ccaggcatcaaataaaacgaaaggc-3'
F_RFP_pl.b
5'-GTGCTAGTGTAGATCGCccaggcatcaaataaaacgaaaggc-3'
F_YFP_pl.b
5'-GAGCTGTACAAGTAATAAccaggcatcaaataaaacgaaaggc-3'
R_pl.b
5'- ctagtatttctcctctttctctagtagtgc-3'
R_tat_pl.b
5'- GAGATCGTTATTGTTCATctagtatttctcctctttctctagtagtgc-3'
Sequencing Primers
F_sequence
5'-tgccacctgacgtctaagaa-3'
R_sequence
5'-attaccgcctttgagtgagc-3'