Team:NTNU-Trondheim/Primers

From 2013.igem.org

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<div id="top-section3"><center><img src="https://static.igem.org/mediawiki/2013/5/55/Logo-ntnu.png" alt="header" border="0"  usemap="#igemmap" width="1000" height="400"></center></div>
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         <li><a href='https://2013.igem.org/Team:NTNU-Trondheim/Experiments_and_Results'><span>Experiments and Results</span></a></li>
         <li><a href='https://2013.igem.org/Team:NTNU-Trondheim/Experiments_and_Results'><span>Experiments and Results</span></a></li>
         <li><a href='https://2013.igem.org/Team:NTNU-Trondheim/Parts'><span>BioBrick Parts</span></a></li>
         <li><a href='https://2013.igem.org/Team:NTNU-Trondheim/Parts'><span>BioBrick Parts</span></a></li>
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         <li class='last'><a href='https://2013.igem.org/Team:NTNU_Trondheim/Acknowledgements'><span>Acknowledgements</span></a></li>
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         <li class='last'><a href='https://2013.igem.org/Team:NTNU-Trondheim/Acknowledgements'><span>Acknowledgements</span></a></li>
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Latest revision as of 20:59, 4 October 2013

Trondheim iGEM 2013

header
Mercury
Primers



tat-signal sequence
Primer Sequence
F_pl.b_tat
5'- AGAGAAAGAGGAGAAATACTAGatggccaataacgatctctttcaggcatcacg-3'
F_prefix_tat
5'- GAATTCGCGGCCGCTTCTAGatggccaataacgatctctttcaggcatcacg-3'
R_tat
5'-cgcttgcgccgcagtcgcacgtcg-3'
R_GFP_tat
5'-CTTCTCCTTTACGCATcgcttgcgccgcagtcgcacgtcg -3'
R_CFP_tat
5'-GCCCTTGCTCACCATcgcttgcgccgcagtcgcacgtcg-3'
SYFP
F_I_syfp
5'-GGTGGATCCGGTGGATCAGGTGGATCCGGTGGTAGTatggttagcaagggcgaaga-3'
R_syfp
5'-GCCTTTCGTTTTATTTGATGCCTGGttattatttatacagctcatccatacccaggg-3'
BFP
F_tat_BFP
5'- CGACGTGCGACTGCGGCGCAAGCGatgagcgaactgatcaaagag-3'
R_I_bfp
5'-ACTACCACCGGATCCACCTGATCCACCGGATCCACCattcagtttatgacccagcttgc-3'
GFP
F_tat_GFP
5'-CGACGTGCGACTGCGGCGCAAGCGatgcgtaaaggagaagaact-3'
R_I_gfp
5'-ACTACCACCGGATCCACCTGATCCACCGGATCCACCtttgtatagttcatccatgcc-3'
RFP
F_I_rfp
5'- GGTGGATCCGGTGGATCAGGTGGATCCGGTGGTAGTatggcttcctccgaagacg-3'
R_pl.b_rfp
5'- GCCTTTCGTTTTATTTGATGCCTGGgcgatctacactagcactatcagcg-3'
GFPmut3
F_tat_GFPmut3
5'-CGACGTGCGACTGCGGCGCAAGCGagaaaaggagaagaacttttcactgg-3'
R_pl.b_GFPmut3
5-'GCCTTTCGTTTTATTTGATGCCTGGttatttgtatagttcatccatgcc-3'
CFP
F_tat_CFP
5'-CGACGTGCGACTGCGGCGCAAGCGatggtgagcaagggcgaggagctgttc-3'
R_l_CFP
5'- ACTACCACCGGATCCACCTGATCCACCGGATCCACCcttgtacagctcgtccatgccgagag-3'
YFP
F_l_YFP
5'-GGTGGATCCGGTGGATCAGGTGGATCCGGTGGTAGTatggtgagcaagggcgaggagctgttc-3'
R_pl.b_YFP
5'- GCCTTTCGTTTTATTTGATGCCTGGttattacttgtacagctcgtccatgccg-3'
Protein G
F_pl.b_PrG
5'-AGAGAAAGAGGAGAAATACTAGatggaaaaagaaaaaaaggtaaaatac-3'
F_tat_PrG
5'-CGACGTGCGACTGCGGCGCAAGCGgttgactcaccaatcgaagatacccc-3'
F_pl.b_pelB_PrB

5'-AGAGAAAGAGGAGAAATACTAGATGAAATACCTGCTGCCGACCGCTGCTGCTGGTCTGCTGCTCCTCGCTGCCCAGCCGGCGATGGCC

gttgactcaccaatcgaagatacccc-3'

R_pl.b_PrG
5'- GCCTTTCGTTTTATTTGATGCCTGGgatcggcatattgaaaaggc-3'
R_pl.b_PrGstop
5'-GCCTTTCGTTTTATTTGATGCCTGGttagtcttctttacgttttgaagcgac-3'
Plasmid backbone
F_plasmid
5'-ccaggcatcaaataaaacgaaaggc-3'
F_RFP_pl.b
5'-GTGCTAGTGTAGATCGCccaggcatcaaataaaacgaaaggc-3'
F_YFP_pl.b
5'-GAGCTGTACAAGTAATAAccaggcatcaaataaaacgaaaggc-3'
R_pl.b
5'- ctagtatttctcctctttctctagtagtgc-3'
R_tat_pl.b
5'- GAGATCGTTATTGTTCATctagtatttctcctctttctctagtagtgc-3'
Sequencing Primers
F_sequence
5'-tgccacctgacgtctaagaa-3'
R_sequence
5'-attaccgcctttgagtgagc-3'