Team:Nevada/Notebook/Month4

From 2013.igem.org

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==12 August 5th – August 11th==   
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{{:Team:Nevada/Templates/header}}
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==August 5th – August 11th==   
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Becca, Christian, and Jasmine started a culture for TOP10 cells to be used in second assay. They also purified Lyz103 and KZ144 endolysin.  
Becca, Christian, and Jasmine started a culture for TOP10 cells to be used in second assay. They also purified Lyz103 and KZ144 endolysin.  
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Jon induced LMG194 and BL21 cells containing cell surface fluorescent markers  
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Jon induced LMG194 and BL21 cells containing cell surface fluorescent markers  
Thursday  
Thursday  
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Jon began growing large culture of Plac-INPN-YFP containing BL21 cells for assay
Jon began growing large culture of Plac-INPN-YFP containing BL21 cells for assay
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==August 12th – August 18th== 
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Monday 
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Christian grew RFP containing bacteria cells to be used in an assay.
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Tuesday
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Christian and Jasmine Worked on The RFP assay.
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Wednesday
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Christian and Jasmine observed the data for assay.
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Thursday
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Everyone prepared for poker night
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Friday
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The team had the Poker Night fundraiser
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==August 19th – August 25th== 
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Monday
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Christian did a transformation n for Cody’s submission plasmid. He also started a small culture of TOP 10 cells for assay.
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Tuesday
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Christian performed the assay on the top 10 cells and used protease inhibitor this time. Christian also did a transformation for Jon’s part.
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Christian cleaned and organized the lab.
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Wednesday
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Christian dissolved pBAD and Xanthamonus endolysin primers. He also examined assay results and cleaned the lab.
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Thursday
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Jon cleaned lab
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Friday
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Christian grew cells for assay
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Saturday
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Christian performed assay.
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Sunday
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==August 26th – September 1st==
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Monday
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First day of school for all.
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Christian, Jon, and Jasmine started organizing the citations for the papers used in this project. They also worked on the abstract.
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Tuesday
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Christian, Jon, and Jasmine worked on abstract and paper citations.
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Wednesday
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Christian started a small colony for the assay.
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Thursday
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Christian and Jasmine did another assay with lysozyme with Pseudomonas aeruginosa cells
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Friday
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Christian cleaned labs, threw away old buffers, and washed dishes.
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Saturday
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Christian and Jasmine worked on assay. 
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==September 2nd – 8th==
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Monday
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Christian grew cells for assay
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Tuesday
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Christian did the assay.
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Wednesday
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Thursday
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Becca, Christian, and Jasmine did a lysozyme assay (but in eppendorf tubes) with Pseudomonas aeruginosa and got successful results.
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Friday 
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Becca, Christian, Dafne, Jon, and Jasmine discussed our project and financial situation with the provost.
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Saturday 
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Sunday
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==September 9th – 15== 
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Monday
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Tuesday
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Christian, Behdad, Jasmine, and Jon worked on finishing the submission plasmid portion of the project.
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Wednesday
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Christian, Behdad, Jasmine, and Jon worked on finishing the submission plasmid portion of the project.
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Thursday
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Christian, Behdad, Jasmine, and Jon worked on finishing the submission plasmid portion of the project.
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Friday
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Christian, Behdad, Jasmine, and Jon worked on finishing the submission plasmid portion of the project.
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Saturday 
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The team participated in the college FIELD DAY. Dafne ate dippin dots
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Sunday
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==September 16th – 22==
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Monday
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Christian, Behdad, Jasmine, and Jon worked on finishing the submission plasmid portion of the project.
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Tuesday
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Christian, Behdad, Jasmine, and Jon worked on finishing the submission plasmid portion of the project.
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==September 23rd -29th== 
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Monday
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Behdad, Christian, Dafnne, jasmine, and Jon worked on the website content.
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Tuesday
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Behdad, Christian, Dafnne, jasmine, and Jon worked on the website content.
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Wednesday
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Behdad, Christian, Dafnne, jasmine, and Jon worked on the website content.
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Thursday
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Behdad, Christian, Dafnne, jasmine, and Jon worked on the website content.
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Friday
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Behdad, Christian, Dafnne, jasmine, and Jon worked on the website content.

Latest revision as of 03:54, 28 September 2013