Team:Osaka/Human practice

From 2013.igem.org

(Difference between revisions)
Line 24: Line 24:
</style>
</style>
<div><div id="safety" style=" width:800px;">
<div><div id="safety" style=" width:800px;">
-
<p class="aaaa2">Although we did not encounter any issues with biosafety during the course of this project, a discussion of safety is mandated by the iGEM requirements so we included it here.</p>
+
 
-
<br>
+
-
<br>
+
<p class="aaaa">
<p class="aaaa">
-
Q1. Would any of your project ideas raise safety issues in terms of researcher safety, public safety or environmental safety ?</p>
+
About E.col-Interior
-
<br>
+
 
-
<p class="aaaa">
+
With this kit , people can enjoy the beautiful pattern E.coli make.  
-
No, our project ideas do not raise any safety issue in terms of researcher safety, public safety or environmental safety. We use E.coli(K12) strain DH5α, DH10b, DH1, and DNA used in our laboratory were all of safe genes, moreover, our experiment was controlled under P1 level room. They are safe for the researcher and the environment, and they do not have any hazard outside the lab.</p>
+
Then maybe you wonder why E.coli can make patterns.
-
<br><br>
+
Actually the condition of the plate (agar gel) is a bit special.
-
<p class="aaaa">
+
It is hard (not as soft as usual plate) and there is little nutrition on it.
-
Q2. Can your project auses risks to security through malicious misuse by individuals, groups, or countries?</p>
+
So E.colis differ slowly consuming nutrition. And when nutrition around the front line of the colony is little , E.coli have to wait for nutrition coming from far away.
-
<br>
+
And when nutrition came to the front line , E.coli consume it immediately. And as the nutrition is not enough for all the E.coli , there is “E.coli which can catch and consume nutrition” and “E.coli which cannot catch nor consume” .
-
<p class="aaaa">
+
So , the first type of E.coli grow more and more though the second type cannot grow so much.
-
No, within the compass of our imagination, our project ideas cannot be used for some malicious misuse.</p>
+
This is why we can see a “branch-like” patterns.
-
<br><br>
+
And theoretically , we can get this pattern by computer simulation of differential equations. And , the fractal dimension of the “branch-like” colony is almost always 1,72.
-
<p class="aaaa">
+
We hope that many people are interested not only in the beauty of the patterns but also the biological ,physical and mathematical beauty of the mechanism.
-
Q3, If your project moved from a small-scale lab study to become widely used as a commercial/industrial product, what new risks might arise?</p>
+
</p>
-
<br>
+
-
<p class="aaaa">
+
-
Our project idea contains drug resistance gene. So if our project ideas widely used in society, they can accelerate the evolution of the drug-resistant bacterium.</p>
+
-
<br><br>
+
-
<p class="aaaa">
+
-
Q4, Does your project include any design features to address safety risks?</p>
+
-
<br>
+
-
<p class="aaaa">
+
-
In our project, we use –ilvE E.coli strain which cannot produce isoleucine. In addition to that, we have construct apart that codes ilvE in biobrick format. They canbe used to construct an auxotrophic chassis system to prevent bio-hazard.</p>
+
-
<br><br>
+
-
<p class="aaaa">
+
-
Q5, What safety training have you received?</p>
+
-
<br>
+
-
<p class="aaaa">
+
-
Our faculty adviser Prof. Kakimoto gave us safety training. It was about how we safely conduct experiments in a lab, and how we treat and dispose genetically engineered orginisms to prevent bio-hazard.</p>
+
-
<br><br>
+
-
<p class="aaaa">
+
-
Q6, Does your institution have an Institutional Biosafety Committee, or an equivalent group? If yes, have you discussed your project with them? Describe any concerns they raised with your project, and any changes you made to your project plan based on their review.</p>
+
-
<br>
+
-
<p class="aaaa">
+
-
Yes, our university has a biosafety committee which requires students and faculty alike to take a biosafety course as well as submit an application before engaging in any research activity involving genetically-modified organisms. In general, as we stuck to the 'safe' organisms of E. coli , the committee did not show any objection to our project. Here is the link to biosafety guidelines of our institution. ( http://www.osaka-u.ac.jp/jp/about/kitei/reiki_honbun/u035RG00000478.html )</p>
+
</div>
</div>
</div>
</div>

Revision as of 18:04, 27 September 2013

team team team team team team team team
team

About E.col-Interior With this kit , people can enjoy the beautiful pattern E.coli make. Then maybe you wonder why E.coli can make patterns. Actually the condition of the plate (agar gel) is a bit special. It is hard (not as soft as usual plate) and there is little nutrition on it. So E.colis differ slowly consuming nutrition. And when nutrition around the front line of the colony is little , E.coli have to wait for nutrition coming from far away. And when nutrition came to the front line , E.coli consume it immediately. And as the nutrition is not enough for all the E.coli , there is “E.coli which can catch and consume nutrition” and “E.coli which cannot catch nor consume” . So , the first type of E.coli grow more and more though the second type cannot grow so much. This is why we can see a “branch-like” patterns. And theoretically , we can get this pattern by computer simulation of differential equations. And , the fractal dimension of the “branch-like” colony is almost always 1,72. We hope that many people are interested not only in the beauty of the patterns but also the biological ,physical and mathematical beauty of the mechanism.

Retrieved from "http://2013.igem.org/Team:Osaka/Human_practice"