"
Team:Paris Bettencourt/Notebook/Trojan Horse/Monday 12th August.html
From 2013.igem.org
(Difference between revisions)
(Created page with "<html> <div class ="tbnote"> <h2>Trojan Horse</h2> <a href="https://2013.igem.org/Team:Paris_Bettencourt/Project/Trojan_Horse" target="_blank" class="tbnotelogo THlogo"> ASDF </a>...") |
|||
| Line 7: | Line 7: | ||
<p><b><em> | <p><b><em> | ||
<!-- === Modify from here === --> | <!-- === Modify from here === --> | ||
| - | + | ||
<!-- === To here === --> | <!-- === To here === --> | ||
</br></em></b></p> | </br></em></b></p> | ||
| Line 29: | Line 29: | ||
iT.0002 (Rv, added BglII cutsite) AGATCTGTGAGTTAGCTCACTCATTAGG | iT.0002 (Rv, added BglII cutsite) AGATCTGTGAGTTAGCTCACTCATTAGG | ||
</p> | </p> | ||
| + | <img src="https://static.igem.org/mediawiki/2013/7/7a/ParisB2013TH_12_08.jpg" width="200" height="250"> | ||
<p lang="en-US"> | <p lang="en-US"> | ||
Column 1; 100kb plus ladder | Column 1; 100kb plus ladder | ||
Latest revision as of 09:16, 3 October 2013
Trojan Horse
ASDF12th August
Clovis
Start 2 cultures of MG1655 pACYCDuet-1 strain (ST003), 5mL each for further miniprep
Phusion PCR of lacZalpha out of pUC18 (pT007)
with primers :
iT.0001 (Fw, added NcoI cut site) CCATGGCTGGCTTAACTATGCGG
iT.0002 (Rv, added BglII cutsite) AGATCTGTGAGTTAGCTCACTCATTAGG
Column 1; 100kb plus ladder
Column 2,3,4,5,6; PCR product
Gel purification of column 5 and 6 → 19,2 ng/ul
