Team:Paris Bettencourt/Notebook/Trojan Horse/Monday 12th August.html
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Revision as of 12:33, 20 September 2013
Trojan Horse
ASDF12th August
Place your twit here
Clovis
Start 2 cultures of MG1655 pACYCDuet-1 strain (ST003), 5mL each for further miniprep
Phusion PCR of lacZalpha out of pUC18 (pT007)
with primers :
iT.0001 (Fw, added NcoI cut site) CCATGGCTGGCTTAACTATGCGG
iT.0002 (Rv, added BglII cutsite) AGATCTGTGAGTTAGCTCACTCATTAGG
Column 1; 100kb plus ladder
Column 2,3,4,5,6; PCR product
Gel purification of column 5 and 6 → 19,2 ng/ul