Team:Paris Bettencourt/Notebook/Trojan Horse/Monday 12th August.html

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Trojan Horse

12th August


Clovis

Start 2 cultures of MG1655 pACYCDuet-1 strain (ST003), 5mL each for further miniprep

Phusion PCR of lacZalpha out of pUC18 (pT007)

with primers :

iT.0001 (Fw, added NcoI cut site) CCATGGCTGGCTTAACTATGCGG

iT.0002 (Rv, added BglII cutsite) AGATCTGTGAGTTAGCTCACTCATTAGG

Column 1; 100kb plus ladder

Column 2,3,4,5,6; PCR product

Gel purification of column 5 and 6 → 19,2 ng/ul

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