Team:SDU-Denmark

From 2013.igem.org

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     <h2 class="onBlack">Doesn't rubber come from trees?</h2>
     <h2 class="onBlack">Doesn't rubber come from trees?</h2>
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Let your eyes (and mouse) wander to these trees to discover our idea on how to help the environment and change the future of rubber-production. Take a look at our short <strong>project description</strong> below.
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Let your eyes (and mouse) wander to these trees to discover our ideas on how to help the environment and change the future of rubber production. Take a look at our short <strong>project description</strong> below.
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The growing demand for natural rubber causes deforestation of the rainforest or occupation of arable lands, all due to the founding of new plantations. If producing rubber by bacteria succeeds, production of natural rubber will not be limited to the regions where the rubber tree can grow but can be done even in barren lands.  
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The growing demand for natural rubber causes deforestation of the rainforest and occupation of arable lands, due to the establishment of new plantations. If producing rubber by bacteria succeeds, production of natural rubber will not be limited to the regions where the rubber tree can grow. Rather, rubber can be produced even in barren lands.  
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Our project aims to make a common bacteria able to produce natural rubber while grown under controlled conditions.  
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Our project aims to enable a common bacteria to produce natural rubber while grown under controlled conditions.  
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Natural rubber is composed of long molecules, each consisting of the substance IPP linked together like a chain. The common bacteria that we use already possesses the ability to produce the IPP, but it lacks the enzyme to connect the IPP links together into a chain.  
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Natural rubber is composed of molecules consisting of the substance IPP linked together like a chain. The common bacteria that we use (E. coli) already possesses the ability to produce the IPP, but it lacks the enzyme to connect the IPP links together into a chain.  
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Through this project we introduce the enzyme that the rubber tree has for connecting the links, into the bacteria. Furthermore we manipulate the bacteria into producing more of the IPP links.  
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We introduced the enzyme that the rubber tree has for connecting the links into the bacteria. Furthermore, we introduced genes that allow the bacteria further production of the IPP links.  
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     <h2 class="onBlack">Status as of 6th of august 2013</h2>
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     <h2 class="onBlack">No, rubber is made in the lab.</h2>
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      Not much free time, but our summer has definitely been exciting so far...
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If you wish to see how, click here to start the interactive tour.
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Currently we have finished two subprojects. We have cloned two genes, <i>dxs</i> from another bacteria  and IspG, into the <i>E. coli</i>. These genes are the limiting steps in the synthesis of IPP and are introduced into the bacteria to optimise the IPP production. Furthermore we have sequenced the <i>dxs</i> gene of <i>E. coli</i> in order to improve a preexisting biobrick.
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Just days before the deadline of iGEM 2013, we got the peaks that our entire summer built towards: Two small bumps on the NMR indicating the presence of bacterially produced rubber in our strain of E. coli. With a mad scramble to the finish line, our initial indication was reinforced. Click anywhere along this text to start the interactive tour, which will guide you along the path to rubber.
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Latest revision as of 02:46, 5 July 2014

Doesn't rubber come from trees?

Let your eyes (and mouse) wander to these trees to discover our ideas on how to help the environment and change the future of rubber production. Take a look at our short project description below.

The growing demand for natural rubber causes deforestation of the rainforest and occupation of arable lands, due to the establishment of new plantations. If producing rubber by bacteria succeeds, production of natural rubber will not be limited to the regions where the rubber tree can grow. Rather, rubber can be produced even in barren lands.

Our project aims to enable a common bacteria to produce natural rubber while grown under controlled conditions. Natural rubber is composed of molecules consisting of the substance IPP linked together like a chain. The common bacteria that we use (E. coli) already possesses the ability to produce the IPP, but it lacks the enzyme to connect the IPP links together into a chain. We introduced the enzyme that the rubber tree has for connecting the links into the bacteria. Furthermore, we introduced genes that allow the bacteria further production of the IPP links.