Team:SDU-Denmark/Tour13

Attributions

Showing gratitude to those we could not have done without

"I get by with a little help from my friends." - John Lennon

Sponsors

We would like to thank our sponsor University of Southern Denmark for giving us the funds for the project, making it possible for us to participate in iGEM. In particular, we would like to thank Dean Henrik Pedersen and the Faculty of Science at University of Southern Denmark. We would also like to thank Bionordika for sponsoring the USER-enzymes.

Laboratory support

We would like to thank Associate Professor, Ph.D. Jakob Møller Jensen & The Microbiology group, and the rest of the Department of Biochemistry and Molecular Biology for letting us use their lab and equipment for the duration of the project.

• Medical Laboratory Technician Simon Rose helped us tremendously with safety; introducing us to the laboratory as well as aiding with the safety form.
  
• Postdoc Anders Boysen helped troubleshoot when the PCR reactions were misbehaving.
• Postdoc Mikkel Jørgensen gave us a lifesaving course in primer design.
• Academic Assistant Eva Lillebæk answered our questions when needed in the everyday lab.
• Associate Professor, dr. Paul Stein helped us with our characterization through NMR support.
• Lennart Bjerre Nielsen and Pernille Lassen helped us with our characterization through MALDI support.
• Academic Assistant, Ph.D. Lise Junker Nielsen helped us with our rubber purification process.
• Ph.D. Tine Daa Schrøder helped us with our Dynamic Light Scattering measurements.
• Head of Department (Institute of Chemical Engineering, Biotechnology and Environmental Technology), Professor, Lars Porskjær Christensen, Medical Laboratory Technician Hanne V. Hemmingsen, Medical Laboratory Technician Dorthe Lillesø, and Medical Laboratory Technichian Brian Hermansen all helped us with our GC characterization.

General support

• The iGEM team from DTU hosted an iGEM crash course in April. They introduced us to the USER-cloning technique, which we have used for the duration of our project.
• Innovation - & Entrepreneurship Expert Sabina Kethelz held a course in project management, giving us the tools to create a better group dynamic and to tackle any problem that arose.
• Associate Professor, Ph.D. Birthe Vester gave a lecture on antibiotic resistance at the DK meet-up.
• DTU and UNIK-Copenhagen iGEM teams visited us at the DK meet-up and gave us some great feedback at the workshop.
• Bioscientific dissemination at SDU gave us great feedback on our presentation in preparation of the jamboree.
• Stud.cand.scient. Claus Asger Lykkebo did an amazing job, helping us designing and creating our own logo.

Technical support

• Associate Professor Kristian Debrabant, helped with the some of the technical programming for the modeling of our system.
• Systems developer, datamatician Kenneth Jensen helped us a great deal with the creation of our wiki, teaching us how to create a userfriendly site. He also helped with the more complex programming, such as the tour menu, as well as helping with general layout and design issues.ZeusSuperman

Litterature sources

The Team:
Homepage of University of Southern Denmark. (Link)
Odense Central Local History Library and City Archives. (Link)

The rubber issue:
Tanaka, Y., & J. T. Sakdapipanich. 2001. Chemical structure and occurrence of natural polyisoprenes, p. 1-25. In T. Koyama & A. Steinbüchel (ed.), Biopolymers, vol. 2. Polyisoprenoids. Wiley-VCH, Weinheim, Germany.
(Link)
K.O. Omokhafe & J.E. Alika: Clonal stability of latex yield in eleven clones of Hevea brasiliensis Muell. Arg. Gen Mol Biol 2003, 26:313-317.
The International Rubber StudyGroup IRSG (Link)

Current situation:
The International Rubber StudyGroup IRSG (Link)

• International Smallholder Rubber Conference Phnom Penh, Cambodia 24 June 2009 Panel 4 : Prospects - IRSG
• Rubber Statistical Bulletin, April - June 2013 edition by IRSG

Threadingham, D., et al Synthetic rubber. In Ullmann's Encyclopedia of Industrial Chemistry, Bohnet, M., et al. (eds.), Wiley-VCH, Weinheim, 2006, Vol. A23 pp. 53- 59, 93-97.
The Great Danish Encyclopedia (Link)
K.O. Omokhafe & J.E. Alika: Clonal stability of latex yield in eleven clones of Hevea brasiliensis Muell. Arg. Gen Mol Biol 2003, 26:313-317.

The fatal future:
Li, H., et al: Demand for rubber is causing the loss of high diversity rain forest in SW China. Biodivers Conserv (2007) 16:1731–1745
UN 2300 report (Link)

Specifications:
Lishan Zhao, Wei-chen Chang, Youli Xiao, Hung-wen Liu and Pinghua Liu: Methylerythritol Phosphate Pathway of Isoprenoid Biosynthesis, Annu. Rev. Biochem. 2013. 82:497-530, page 500.
Alaxander Steinbüchel: Production of rubber-like polymers by microorganism, Current Opinion in Microbiology 2003. 6:261-270. (book)

Modelling: